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Purified anti-NF-κB p100/p52 Antibody

Pricing & Availability
Clone
W16082A (See other available formats)
Regulatory Status
RUO
Other Names
DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16082A_PURE_NF-kappaB2_Antibody_0_WB_030617
Total cell lysates (15 µg total protein) from A431, HaCat, THP-1, and HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NF-κB p100/p52 Antibody, clone W16082A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
  • W16082A_PURE_NF-kappaB2_Antibody_0_WB_030617
    Total cell lysates (15 µg total protein) from A431, HaCat, THP-1, and HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NF-κB p100/p52 Antibody, clone W16082A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
  • W16082A_PURE_NF-kappaB2_Antibody_1_WB_030617
    Total cell lysates (15 µg protein) from THP-1, HeLa and NIH3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti- NF-κB2 antibody (clone W16082A) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the molecular weight ladder.
  • W16082A_PURE_NF-kappaB2_Antibody_IF_010818
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.0 and 0.5 µg/mL anti- NF-κB2 antibody (clone W16082A) overnight at 4°C followed by Alexa Fluor™ 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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695902 100 µg 276 CHF
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Description

Nuclear factor NF-kappa-B p100/p52 subunit (NF-κB2) is a member of the Rel/NF-κB family of transcription factors. NF-κB2 p100 subunit contains an ankyrin repeat domain that has IκB activity to retain NF-κB2 proteins in the cytoplasm. Upon activation, NF-κB2 p100 subunit is processed to p52 subunit through the ubiquitin-proteasome pathway and translocated to the nucleus. NF-κB2 p52 subunit forms homo- or heterodimers with other Rel-like domain-containing proteins, including c-REL, RELA/p65, RELB, NFKB1/p105, and NFKB1/p50, subsequently binds to specific kappa-B sites and regulates expression of their target genes. NF-κB participates in the regulation of peripheral lymphoid development, thymic development, and B cell maturation. Mutations in the NF-κB2 gene lead to defects in B cell development and have been associated with lymphoma and other hematogenous malignancies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human NF-κB2 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W16082A does not cross-react with mouse (in-house tested).

RRID
AB_2650935 (BioLegend Cat. No. 695902)

Antigen Details

Structure
NF-κB2 p100 subunit contains 900 amino acids with a predicted molecular weight of 96.7 kD. Contains an N-terminal RHD domain for DNA binding and a C-terminal ankyrin repeat domain. NF-κB2 p100 subunit is processed to a p52 subunit upon activation.
Distribution

Nucleus and cytoplasm.

Function
NF-κB2 is a transcription factor involved in the regulation of peripheral lymphoid development, thymic development, and B cell maturation.
Interaction
Forms homodimers or heterodimers with other Rel-like domain-containing proteins including c-REL, RELA/p65, RELB, NFKB1/p105, and NFκB1/p50. Interacts with BCL3, BTRC, TSC22D3, and MAP3K8.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lee CE, et al. 2014. Blood. 124:2964.
2. Savinova OV, et al. 2009. Mol Cell. 34:591.
3. Tucker E, et al. 2007. J. Immunol. 179:7514.
4. Kim KE, et al. 2000. Oncogene. 19:1334.
5. Heusch M, et al. 1999. Oncogene. 18:6201.
6. de Wit H, et al. 1998. Leukemia. 12:363.

Gene ID
4791 View all products for this Gene ID
UniProt
View information about NF-kappaB p100/p52 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 03.20.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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