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Purified anti-NFATc2 Antibody

Pricing & Availability
Clone
16A12A31 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 (NFATc2), T-cell transcription factor NFAT1, NFAT pre-existing subunit (NFATp)
Isotype
Mouse IgG1, κ
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Product Citations
publications
16A12A31_Purified_NFATc2_Antibody_WB_122117
Total lysates (15 µg protein) from Raw 264.7 (Lane 1) and Jurkat (Lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL purified anti-NFATc2 antibody, clone 16A12A31 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-NFATc2 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder
  • 16A12A31_Purified_NFATc2_Antibody_WB_122117
    Total lysates (15 µg protein) from Raw 264.7 (Lane 1) and Jurkat (Lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL purified anti-NFATc2 antibody, clone 16A12A31 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-NFATc2 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder
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658402 100 µg 265 CHF
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Description

NFATc2 is a family member of the NFAT (calcineurin-dependent nuclear factor of activated T cells) transcription factors, regulating expression of the genes involved in T-cell activation and proliferation. In resting T cells, NFATc2 protein is phosphorylated and is present in the cytoplasm. Upon stimulation, NFATc2 protein is dephosphorylated by calcium dependent phosphatase, resulting in the translocation of NFATc2 protein to the nucleus, where it induces expression of target genes. In addition to its roles in immune cells, NFATc2 is revealed to mediate VEGF signaling, transactivating the gene encoding cyclooxygenase 2 (COX2). The production of prostaglandins by cycloxoygenase 2 is required for the migration of endothelial cells and angiogenesis. NFATc2 is also suggested to participate in tumorigenesis and invasive migration of tumor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal Peptide-KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 4.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562881 (BioLegend Cat. No. 658402)

Antigen Details

Structure
925 amino acids, predicted molecular weight of 100 kD; contains two transactivation domains and a Rel homology domain (RHD) responsible for DNA binding
Distribution

Phosphorylated form in cytoplasm; translocates to the nucleus when dephosphorylated

Function
Transcription factor, regulating T cell development, differentiation, and activation
Interaction
Forms complex with other family members; interacts with p300/CBP, MAF, GATA4, and NFATC2IP
Cell Type
Mesenchymal Stem Cells, Embryonic Stem Cells, B cells, Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Gerlach K, et al. 2012. Cancer Res. 72:4340.
2. Ohoka Y, et al. 2011. J. Immunol. 186:733.
3. Nguyen TN, et al. 2010. Mol. Immunol. 47:2314.
4. Alev C, et al. 2009. Cell Cycle. 8:1803.
5. Becker M, et al. 2009. J. Immunol. 182:6136.
6. Horsley V, et al. 2002. J. Cell Biol. 156:771.

Gene ID
4773 View all products for this Gene ID
UniProt
View information about NFATc2 on UniProt.org
Go To Top Version: 1    Revision Date: 11.26.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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