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Purified anti-NFATC3 Antibody

Pricing & Availability
Clone
W15221A (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor of activated T-cells 3, NFAT4, NFATX
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15221A_PURE_NFATC3_Antibody_WB_040419_updated.png
Total cell lysates (15 µg total protein) from Jurkat, MCF-7, HeLa, and NIH3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NFATC3 Antibody, clone W15221A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W15221A_PURE_NFATC3_Antibody_WB_040419_updated.png
    Total cell lysates (15 µg total protein) from Jurkat, MCF-7, HeLa, and NIH3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NFATC3 Antibody, clone W15221A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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690602 100 µg 253 CHF
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Description

Nuclear factor of activated T cells C3 (NFATC3), also known as NFAT4 or NFATx, is a member of the NFAT family of transcription factors. Several NFATC3 variants generated by alternative splicing were identified. NFATC3 plays an important role in the regulation of T cell activation-related gene expression, especially in the induction of IL-2.  NFATC3 is expressed in immune cells, such as double positive (DP) thymocytes, mature CD4+ T cells, and mast cells. It is also found in non-immune cells such as smooth muscle and Schwann cells. In the resting T cells, NFATC3 is located in the cytoplasm and is phosphorylated. Upon T cell activation by TCR stimulation, the increase of intracellular Ca2+ induces NFATC3 dephosphorylation via calcineurin. Dephosphorylated NFATC3 exposes the nuclear localization signal and is subsequently translocated from the cytosol to the nucleus. Through the highly conserved Rel-like binding domain, NFATC3 associates with specific consensus DNA sequences and regulates gene expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human NFATC3 recombinant protein (826-1075 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W15221A may react with isoforms 1-4 (MW 112.6~115.6). NFATs are heavily phosphorylated proteins, so the observed MWs of NFATC3 can be higher than predicted.

RRID
AB_2629823 (BioLegend Cat. No. 690602)

Antigen Details

Structure
There are 6 reported isoforms of NFATC3 (MWs: 115.1, 115.2, 115.6, 112.6, 77.2, and 80.6 kD). The protein contains a RHD (Rel-like) domain responsible for DNA binding.
Distribution

Cytoplasm and nucleus.

Function
NFATC3 is a component of activated T cells DNA-binding transcription complex, regulating gene expression in immature thymocytes and T cells.
Interaction
Forms a multicomponent complex with NFATC1 and NFATC2. Interacts with Raf-1.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Ran Y, et al. 2014. J. Recept. Signal. Transduct. Res. 34:270.
2. Geil WM, et al. 2014. FEBS J. 281:1170.
3. Neria F, et al. 2013. Glia. 61:1052.
4. Urso K, et al. 2011. Blood. 118:795.
5. Canté-Barrett K, et al. 2007. J. Immunol. 179:103.
6. Chen J, et al. 2003. J. Immunol. 170:3109.

Gene ID
4775 View all products for this Gene ID
UniProt
View information about NFATC3 on UniProt.org
Go To Top Version: 3    Revision Date: 04.04.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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