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Purified anti-Nicastrin Antibody

Pricing & Availability
Clone
9C3 (See other available formats)
Regulatory Status
RUO
Other Names
NCSTN
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
9C3_PURE_Nicastrin_Antibody_1_WB_032318
Western blot of anti-Nicastrin antibody (clone 9C3) and isotype-matched control mouse IgG2b. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blots were incubated with 0.5 μg/mL of anti-Nicastrin or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. In human the antibody also detects the deglycosylated nicastrin.
  • 9C3_PURE_Nicastrin_Antibody_1_WB_032318
    Western blot of anti-Nicastrin antibody (clone 9C3) and isotype-matched control mouse IgG2b. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blots were incubated with 0.5 μg/mL of anti-Nicastrin or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. In human the antibody also detects the deglycosylated nicastrin.
  • 9C3_PURE_Nicastrin_Antibody_2_IHC-P_032318
    IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded human breast cancer tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 9C3_PURE_Nicastrin_Antibody_3_IHC-P_032318
    IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 9C3_PURE_Nicastrin_Antibody_4_IHC-P_032318
    IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
852301 25 µg 112 CHF
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852302 100 µg 276 CHF
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Description

Nicastrin is one of the essential components of the γ-secretase complex. The γ-secretase complex is one of the proteases involved in processing amyloid precursor protein (APP) to amyloid beta (Aβ). Nicastrin is not catalytically active, instead, it promotes the maturation and localization of other catalytic and regulatory units of the γ-secretase complex. Nicastrin has alsp been reported to regulate neprilysin, which is the enzyme involved in the degradation of Aβ fragment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to the C-terminal 18 a.a. of mouse Nicastrin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

In human, the antibody also detects the deglycosylated nicastrin, which is observed at ~ 78kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Esselens C, et al. 2004. J. Cell Biol. 166(7):1041. (WB)
Product Citations
  1. Aron L, et al. 2023. Nat Commun. 7030:14. PubMed
RRID
AB_2728591 (BioLegend Cat. No. 852301)
AB_2728591 (BioLegend Cat. No. 852302)

Antigen Details

Structure
Deglycosylated nicastrin is a 709 amino acid protein with a molecular mass of ~78 kD. Higher molecular weight band of ~110 kD corresponding to glycosylated nicastrin is observed by WB.
Distribution

Tissue Distribution: Ubiquitously expressed.
Cellular: Distribution: Plasma Membrane, endoplasmic reticulum, lysosome, golgi apparatus, and cytoskeleton.

Function
Nicastrin is an essential component of the γ-secretase complex that processes amyloid precursor protein to amyloid beta. It is also involved in degradation of amyloid beta fragments.
Interaction
Presenilin, APH-1, PEN-2
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
Enzymes and Regulators, Secretases
Antigen References
  1. Yang D, et al. 2002. J. Biol. Chem. 31:28135.
  2. Johnson DS, et al. 2017. Cold Spring Harb Perspect Med. 10.1101/cshperspect.a024067.
  3. Zhang X, et al. 2014. Front Cell Neurosci. 10.3389/fncel.2014.00427.
Gene ID
23385 View all products for this Gene ID
UniProt
View information about Nicastrin on UniProt.org
Go To Top Version: 1    Revision Date: 03.29.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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