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Purified anti-NLRC4 (IPAF) Antibody

Pricing & Availability
Clone
6H9B13 (See other available formats)
Regulatory Status
RUO
Other Names
NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein (CLAN), Caspase recruitment domain-containing protein 12 (CARD12), Ice protease-activating factor (IPAF)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_6H9B13_Purified_NLRC4_Antibody_031519_updated.png
Total cell lysates (15 or 30 µg total protein) from HeLa (negative) and THP-1 (positive) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL Purified anti-NLRC4 Antibody, clone 6H9B13, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_6H9B13_Purified_NLRC4_Antibody_031519_updated.png
    Total cell lysates (15 or 30 µg total protein) from HeLa (negative) and THP-1 (positive) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL Purified anti-NLRC4 Antibody, clone 6H9B13, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_6H9B13_Purified_NLRC4(IPAF)_Antibody_2IF_032614.jpg
    THP-1 cells were stained with purified anti-NLRC4 (6H9B13) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
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659702 100 µg 265 CHF
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Description

NLRC4 , also known as IPAF, is a member of the NOD-like receptor (NLR) family. The NLR proteins share a conserved DNA-binding NACHT domain, a caspase recruitment domain (CARD), and a leucine-rich repeat (LRR) domain responsible for pathogen pattern recognition. NLRC4 was found to play an important role in the innate immune response against bacterial infection. During Gram-negative bacteria infections, such as Shigella, Salmonella, Legionella, or Pseudomonas, NLRC4 detects and is activated by translocated bacterial flagellin or PrgJ-like rod proteins subsequently resulting in the assembly of the NLRC4 inflammasome. The activated inflammasome drives proteolytic activation of caspase-1, leading to the processing and secretion of the proinflamatory cytokines IL-1β and IL-18.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human NLRC4 recombinant protein (1-88 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Baker GJ, et al. 2016. Oncoimmunology. e1163461:5. PubMed
  2. Fytianos K, et al. 2017. ACS Nano. 375:11. PubMed
RRID
AB_2563347 (BioLegend Cat. No. 659702)

Antigen Details

Structure
1024 amino acids, predicted molecular weight of 116 kD. Contains a CARD domain, a NACHT domain, and 14 leucine-rich repeats.
Distribution

Cytoplasm.

Function
NLRC4 is involved in the assembly of the inflammasome complex, activation of pro-caspase-1, and induction of matured proinflammatory cytokines during bacterial infections.
Interaction
Foms homo-oligomer in NLRC4 inflammasome. Interacts with Caspase-1, ASC, NOD2, NALP1, and BCL10.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Cai S, et al. 2012. J. Immunol. 188:5623.
2. Franchi L, et al. 2012. Nat. Immunol. 13:449.
3. Carvalho FA, et al. 2012. Mucosal Immunol. 5:288.
4. Tomalka J, et al. 2011. PLoS Pathog. 7:e1002379.
5. Pereira MS, et al. 2011. J. Immunol. 187:6447.
6. Sauer JD, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:12419.

Gene ID
58484 View all products for this Gene ID
UniProt
View information about NLRC4 on UniProt.org
Go To Top Version: 4    Revision Date: 01.14.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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