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Purified anti-NLRP1 Antibody

Pricing & Availability
Clone
9F9B12 (See other available formats)
Regulatory Status
RUO
Other Names
NLR Family, Pyrin Domain Containing 1, NALP1, Caspase Recruitment Domain-Containing Protein 7 (CARD7), Systemic Lupus Erythematosus, Vitiligo-Related 1 (SLEV1), NAC, CIDED, NALP1, DEFCAP, PP1044, VAMAS1, CLR17.1, DEFCAP-L/S
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_9F9B12_PURE_NLRP1_Antibody_WB_113018
Total cell lysates (15 µg total protein) from MCF7, MOLT4 (negative/low expressers) and Daudi cells (positive control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL of Purified anti-NLRP1 Antibody, clone 9F9B12, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 1_9F9B12_PURE_NLRP1_Antibody_WB_113018
    Total cell lysates (15 µg total protein) from MCF7, MOLT4 (negative/low expressers) and Daudi cells (positive control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL of Purified anti-NLRP1 Antibody, clone 9F9B12, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2_9F9B12_PURE_NLRP1_Antibody_WB_083115
    Total cell lysate from human HeLa (lane 1), K562 (lane 2), Jurkat (lane 3), and Raw264.7 (lane 4) cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NLRP1 (clone 9F9B12) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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679802 100 µg 288 CHF
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Description

NLRP1, also known as NALP1, is a member of the NOD-like receptor (NLR) family that is responsible for the recognition of pathogen-associated molecular patterns (PAMP). NLRP1 is a sensor component of inflammasome that is involved in innate immune response. NLRP1-dependent activation of caspase-1 drives rapid proteolytic activation of caspase-1 and the maturation of the proinflammatory cytokines IL-1β and IL-18. The activation of NLRP1 leads to cell death of macrophages via pyroptosis, which results in the release of cytosolic contents and induces inflammatory responses. NLRP1 is found to be expressed at high levels in the brain and plays a crucial role in neuronal cell death and neurodegenerative diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human NLRP1 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Li M, et al. 2021. Oxid Med Cell Longev. 2021:2353504. PubMed
  2. Jenster LM, et al. 2023. J Exp Med. 220: . PubMed
  3. Despic V, et al. 2023. Nature. 614:358. PubMed
  4. Liu L, et al. 2017. J Crohns Colitis. 10.1093/ecco-jcc/jjw219. PubMed
  5. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
RRID
AB_2566263 (BioLegend Cat. No. 679802)

Antigen Details

Structure
1473 amino acids with a predicted molecular weight of 165.8 kD. Contains an N-terminal PYD domain, a C-terminal CARD domain, a NACHT domain, and 6 leucine-rich repeats (LRR).
Distribution

Cytoplasm and nucleus.

Function
NLRP1 mediates cytochrome c-dependent apoptosis. It is a key component of the NLRP1 inflammasome, which leads to the processing and secretion of IL-1β and IL-18.
Interaction
Interacts with caspase-2, caspase-9, APAF1, EIF2AK2, and MEFV.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Tan CC, et al. 2015. J. Neuroinflammation 12:18.
2. Tan MS, et al. 2014. Cell Death Dis. 5:e1382.
3. Cirelli KM, et al. 2014. PLoS Pathog. 10:e1003927.
4. Maier NK, et al. 2014. J. Immunol. 192:763.
5. Levandowski CB, et al. 2013. Proc. Natl. Acad. Sci. USA 110:2952.
6. de Zoete MR, et al. 2012. Immunity 37:950.

Gene ID
22861 View all products for this Gene ID
UniProt
View information about NLRP1 on UniProt.org
Go To Top Version: 1    Revision Date: 08.31.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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