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Purified anti-NOVA1 Antibody

Pricing & Availability
Clone
Poly28608 (See other available formats)
Regulatory Status
RUO
Other Names
RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, Nova-1
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28608_PURE_NOVA1_Antibody_1_061919
Western blot of purified anti-NOVA1 antibody (Poly28608). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28608_PURE_NOVA1_Antibody_1_061919
    Western blot of purified anti-NOVA1 antibody (Poly28608). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28608_PURE_NOVA1_Antibody_2_061919
    IHC staining of purified anti-NOVA1 antibody (Poly28608) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28608_PURE_NOVA1_Antibody_3_061919
    IHC staining of purified anti-NOVA1 antibody (Poly28608) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28608_PURE_NOVA1_Antibody_4_061919
    IHC staining of purified anti-NOVA1 antibody (Poly28608) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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860801 25 µg 125 CHF
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Description

RNA binding-protein Nova-1(NOVA1) is a brain-specific splicing factor well conserved in vertebrates. It was first identified as an antigen in paraneoplastic opsoclonus-myoclonus ataxia (POMA), a disorder related to breast cancer and motor dysfunction. Nova1 binds to a specific sequence cluster in the brain to regulate neuronal pre-mRNA splicing. Nova1-binding sequences are 94% identical in vertebrates, suggesting the binding is sequence dependent. Nova1 null mice die postnatally from motor deficits associated with neuronal cell death in spinal cord and brain stem.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant NOVA1 (Ala295-Gly510) with two N-terminal tags.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Multiple bands observed on WB correspond to different NOVA1 splice variants (1).

RRID
AB_2810742 (BioLegend Cat. No. 860801)

Antigen Details

Structure
Human NOVA1 is a 507 amino acid protein with a predicted molecular mass of ~51 kD.
Distribution

Tissue distribution: Brain
Cellular distribution: Nucleus

Function
NOVA1 regulates RNA-splicing in neurons.
Interaction
Interacts with PTBP2.
Cell Type
Neurons
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience
Molecular Family
Microfilaments
Antigen References
  1. Jelen N, et al. 2007. PLOS Genet. 3(10):e173
  2. Buckanovich RJ, et al. 1997. Mol Cell Biol. 17(6):3194-201.
  3. Jensen KB, et al. 2000. Neuron. 2000. 25(2):359-71.
Gene ID
4857 View all products for this Gene ID
UniProt
View information about NOVA1 on UniProt.org
Go To Top Version: 1    Revision Date: 06.19.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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