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Purified anti-NTAL Antibody

Pricing & Availability
Clone
NAP-07 (See other available formats)
Regulatory Status
RUO
Other Names
LAT2 (linker for activation of T cells family), member 2, LAB
Isotype
Mouse IgG1, κ
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Product Citations
publications
NAP-07_PURE_NTAL_Antibody_1_WB_061518
Cell lysate from Ramos (human, lane 1), Raji (human, lane 2), HeLa (human, lane 3), EL4 (mouse, lane 4) and A20 cell lines (mouse, lane 5) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL of Purified anti-NTAL Antibody (clone NAP-07) (upper). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-mouse-IgG secondary antibody for the anti-NTAL antibody (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel) Antibody. Lane M: Molecular weight ladder.
  • NAP-07_PURE_NTAL_Antibody_1_WB_061518
    Cell lysate from Ramos (human, lane 1), Raji (human, lane 2), HeLa (human, lane 3), EL4 (mouse, lane 4) and A20 cell lines (mouse, lane 5) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL of Purified anti-NTAL Antibody (clone NAP-07) (upper). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-mouse-IgG secondary antibody for the anti-NTAL antibody (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel) Antibody. Lane M: Molecular weight ladder.
  • NAP-07_PURE_NTAL_Antibody_2_IHC_083116
    Formalin-fixed paraffin-embedded human tonsil tissue was stained with NAP-07 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
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627102 100 µg 293 CHF
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Description

Non-T cell activation Linker (NTAL) is a transmembrane adaptor protein with an apparent molecular weight of 30 kD expressed on B cells, natural killer (NK) cells, and myeloid cells. The NTAL protein is found in rafts, which are membrane microdomains that participate in B cell activation. NTAL interacts with a variety of proteins including Grb2, PLC gamma 2, and PLC gamma 1. This gene is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant cytoplasmic domain (91-243 a.a.) of human NTAL.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µl/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations
  1. Brühl H, et al. 2014. J Leukoc Biol. 65:96. PubMed
RRID
AB_2133251 (BioLegend Cat. No. 627102)

Antigen Details

Structure
30 kD double palmitoylated protein gene that consists of 14 exons, and its alternative splicing generates three transcript variants.
Distribution

B, natural killer cells, monocytes, mast cells, and activated T cells.

Function
Involved in FCER1 mediated signaling in mast cells and as a negative regulator of early B cell signaling. May also be involved in CFCGR1 mediated signaling in myeloid cells. Couples activation of these receptors with distal intracellular events through the recruitment of Grb2.
Ligand/Receptor
Membrane microdomains (rafts) of B cells, natural killer (NK) cells, and myeloid cells.
Cell Type
B cells, NK cells, Monocytes, Mast cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Yamasaki S, et al. 2007. Mol. Cell Biol. 27(12):4406-15.
2. Brdicka T, et al. 2002. J. Exp. Med. 196(12):1617-26.
3. Tkaczyk C, et al. 2004. Blood 104(1):207-14.
4. Volna P, et al. 2004. 200(8):1001-13.
5. Lebduska P, et al. 2007. 328(1-2):139-51.

Gene ID
7462 View all products for this Gene ID
UniProt
View information about NTAL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.31.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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