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Purified anti-NTRK2 Antibody

Pricing & Availability
Clone
A15019C (See other available formats)
Regulatory Status
RUO
Other Names
Neurotrophic tyrosine kinase receptor type 2, TrkB, GP145-TrkB
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A15019C_PURE_NTRK2_Antibody_WB_033117
Total protein from SH-SY5Y cell lysates and human, mouse and rat brain lysates (15µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-NTRK2 antibody (clone A15019C) ) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower).
  • A15019C_PURE_NTRK2_Antibody_WB_033117
    Total protein from SH-SY5Y cell lysates and human, mouse and rat brain lysates (15µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-NTRK2 antibody (clone A15019C) ) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower).
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695102 100 µg 276 CHF
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Description

NTRK2 (Neurotrophic Tyrosine Kinase, Receptor, type 2), also known as TrkB (Tropomyosin related kinase B), is one of the members of the neurotrophin receptor family, which include TrkA and TrkC. All three Trk proteins exhibit a conserved subdomain that contains a signal peptide, two cysteine rich domains, a cluster of three leucine rich motifs, and two Ig-like domains in the extracellular portion. It has been shown that Trk receptor-mediated neurotrophic signaling regulates neuron physiology, cell proliferation and survival, axon and dendrite growth and patterning, and synaptic plasticity. The neurotrophins that activate NTRK2 are BDNF (Brain Derived Neurotrophic Factor), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4). BDNF is known to trigger NTRK2 dimerization and auto phosphorylation that leads to the activation of downstream signaling pathways. After the receptor-ligand binding, NTRK2-BDNF complex is internalized and transported from the nerve terminal to the cell body as signaling endosomes to mediate numerous signaling events. It has been demonstrated that activation of NTRK2 can lead to the down regulation of the main potassium-chloride cotransporter KCC2 in neurons, thereby increasing the intracellular Cl- concentration in lamina I neurons. Recently, it was shown that BDNF-NTRK2 signaling may play a role in the pathogenesis of post-traumatic stress disorders and mutations in the NTRK2 gene have been associated with obesity and mood disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant full length of human NTRK2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Optimal concentration of usage is 0.1 - 1 µg/mL for WB. The optimal concentration should be determined by titration for each individual assay of interest

RRID
AB_2686965 (BioLegend Cat. No. 695102)

Antigen Details

Structure
Human NTRK2 has 7 isoforms, and predicted Mw ranges from 35 to 93kDa. It has 11 potential N-glycosylation sites.
Distribution

NTRK2 is found primarily in the nervous system. It also can be found in the pancreas, skeletal muscles, and the kidney. A truncated form of TrkB (missing the tyrosine kinase domain) is found in heart, ovary, and the spleen.

Function
NTRK2, when binded to BDNF, NT-3, and NT-4, is able to exert neuron cells' biological functions such as proliferation, survival, axon, and dendrite growth.
Interaction
Interacts with SHC1, PLCG1 and/or PLCG2, SH2B1 and SH2B2, NGFR, SH2D1A, SQSTM1 and KIDINS220, FRS2.
Ligand/Receptor
BDNF, NT-3 and NT-4.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Soluble Receptors, Protein Kinases/Phosphatase
Antigen References

1. Snider WD. 1994. Cell 77:627.
2. Grace PM, et al. 2014. Nat. Rev. Immunol. 14:217.
3. Neveu I and Arenas E. 1996. J. Cell Biol. 133:631.
4. Green CR, et al. 2013. J. Depress Anxiety S4:006.
5. Gupta VK, et al. 2013. Int. J. Mol. Sci. 14:10122.
6. Pla P, et al. 2014. Front Behav. Neurosci. 8:135.
7. Liu X, et al. 2014. J. Biol. Chem. 289:27571.

Gene ID
4915 View all products for this Gene ID
UniProt
View information about NTRK2 on UniProt.org
Go To Top Version: 0    Revision Date: 04.07.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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