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Purified anti-O-GlcNAc Antibody

Pricing & Availability
Clone
CTD110.6 (See other available formats)
Regulatory Status
RUO
Other Names
O-Linked N-Acetylglucosamine
Isotype
Mouse IgM, κ
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Product Citations
publications
CTD110.6_PURE_OGlcNAc_Antibody_1_IF_082316
Immunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at room temperature, followed by incubation with goat anti-mouse IgM Alexa Fluor® 488 secondary antibody (green). The cells were counterstained with DAPI (blue).
  • CTD110.6_PURE_OGlcNAc_Antibody_1_IF_082316
    Immunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at room temperature, followed by incubation with goat anti-mouse IgM Alexa Fluor® 488 secondary antibody (green). The cells were counterstained with DAPI (blue).
  • CTD110.6_PURE_OGlcNAc_Antibody_2_IHCP_082316
    IHC staining of CTD110.6 on formalin-fixed paraffin-embedded human pancreas tissue. Following sodium citrate antigen retrieval, the primary antibody was incubated for 60 minutes at room temperature at 10 µg/ml. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection with DAB chromogen.
  • Pragnya_Das.jpg
    Paraffin-embedded neonatal mouse lung sectioned and stained with purified anti-O-GlcNAc (green, clone CTD110.6), anti-RAGE (red) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Pragnya Das from Drexel University College of Medicine.
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838004 100 µg 347 CHF
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Description

β-O-linked N-acetylglucosamine (O-GlcNAc) is an abundant post-translational modification occuring on serine and threonine residues. The modification has been detected on a variety of nuclear and cytoplasmic proteins involved in diverse cellular functions. Studies to elucidate the role of O-GlcNAc-ylation have been hampered by the lack of a sensitive, specific assay to identify or purify proteins bearing O-GlcNAc.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.

Additional reported applications (for the relevant formats) include: ELISA2, immunocytochemistry7, immunoprecipitation2,5,6, and Western blotting3,4,7.

Application References

(PubMed link indicates BioLegend citation)
  1. Comer FI, et al. 2001. Anal. Biochem. 293:169.
  2. Hart GW, et al. 1995. Adv. Exp. Med. Biol. 376:115. (ELISA, WB, IP)
  3. Anthonisen EH, et al. 2010. J. Biol. Chem. 285:1607. (WB)
  4. Dias WB, et al. 2009. J. Biol. Chem. 32:21327. (WB)
  5. Wang Z, et al. 2007. Mol. Cell Proteomics 8:1365. (IP)
  6. Kim SJ, et al. 2003. J. Biol. Chem. 43:41890. (IP)
  7. Kneass ZT, Marchase RB. 2004. J. Biol. Chem. 44:45759. (WB, ICC, IF)
Product Citations
  1. Kuo WL, et al. 2021. Diagnostics (Basel). 11:. PubMed
  2. Durst MA, et al. 2019. PLoS One. 14:e0210305. PubMed
  3. Liu Y, et al. 2020. Nat Commun. 4.554166667. PubMed
  4. Basehore SE, et al. 2021. Circ Res. 129:1054. PubMed
  5. Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
RRID
AB_2629520 (BioLegend Cat. No. 838004)

Antigen Details

Structure
1046 amino acids with a molecular weight of 117 kD.
Distribution

Cytosol, mitochondrion, nucleus, plasma membrane, and cytoskeleton.

Function
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc), Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1, Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
Biology Area
Cell Biology, Transcription Factors
Gene ID
8473 View all products for this Gene ID
UniProt
View information about O-GlcNAc on UniProt.org
Go To Top Version: 3    Revision Date: 02.04.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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