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Purified anti-OGT Antibody

Pricing & Availability
Clone
W20094A (See other available formats)
Regulatory Status
RUO
Other Names
O-linked N-acetylglucosaminyltransferase (O-GlcNAc transferase, OGT) subunit p110, UDP-N-acetylglocuosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20094A_PURE_OGT_Antibody_052022
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-OGT (clone W20094A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent Lane M: Molecular weight marker.
  • W20094A_PURE_OGT_Antibody_052022
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-OGT (clone W20094A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent Lane M: Molecular weight marker.
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603551 25 µg 118 CHF
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603552 100 µg 293 CHF
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Description

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT) catalyzes the transfer of N-acetyl glucosamine (GlcNAc) post-translational modification to serine, threonine, and cysteine residues of proteins. Serine and threonine residues are modified by O-glycosidic linkage, while cysteine is modified by S-glycosidic linkage. Since the amino acids utilized for O-GlcNAcylation are identical to those used for protein phosphorylation, OGT competes with kinases for modification of those sites. Numerous functions for GlcNAcylation have been reported in regulating protein-protein interactions, and OGT plays diverse roles in the regulation of the cell cycle, transcription, and cellular stress. Dysregulation of O-GlcNAcylation plays a role in the pathology of Alzheimer’s disease, cancer and diabetes. OGT activity is regulated by glucose availability, and plays a role in regulating glycolysis by glycosylation of phosphofructokinase.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human OGT protein fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not meet passing criteria for ICC and IHC.

RRID
AB_2922635 (BioLegend Cat. No. 603551)
AB_2922635 (BioLegend Cat. No. 603552)

Antigen Details

Structure
OGT is a 1,046 amino acid protein with a predicted molecular mass of 116.9 kD.
Distribution

OGT is expressed in the cytoplasm of most cells.

Function
OGT is an enzyme which catalyzes the attachment of the O-GlcNAc post-translational modification.
Interaction
OGT glycoylates a wide array of proteins including histone H2B, AKT1, and EZH2.
Biology Area
Cell Cycle/DNA Replication, Cell Proliferation and Viability, Protein Synthesis
Molecular Family
Enzymes and Regulators, Protein Modifiers
Antigen References
  1. Canovas B and Nebreda A. 2021. Nat Rev Mol Cell Biol. 22:346-366.
Gene ID
8473 View all products for this Gene ID
UniProt
View information about OGT on UniProt.org
Go To Top Version: 1    Revision Date: 04.28.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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