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Purified anti-P-Cadherin Antibody

Pricing & Availability
Clone
12H6 (See other available formats)
Regulatory Status
RUO
Other Names
Cadherin 3, CDH3, Placental Cadherin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
12H6_PURE_P-Cadherin_Antibody_1_091421.png
Whole cell extracts (15 µg total protein) from A-431, HEK293, MCF7, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-P-Cadherin antibody (clone 12H6) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Lane M: Molecular weight marker.
  • 12H6_PURE_P-Cadherin_Antibody_1_091421.png
    Whole cell extracts (15 µg total protein) from A-431, HEK293, MCF7, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-P-Cadherin antibody (clone 12H6) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Lane M: Molecular weight marker.
  • 12H6_PURE_P-Cadherin_Antibody_2_091421.png
    Whole cell extracts (250 µg total protein) prepared from A31 cells were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400102) or purified anti-P-Cadherin antibody (clone 12H6). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a mouse control antibody. Lane M: Molecular weight marker.
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948401 25 µg 118 CHF
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948402 100 µg 293 CHF
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Description

P-cadherin, or placental-cadherin, belongs to one of the three subclasses in the cadherin family. Cadherins are Ca2+ dependent adherent proteins involved in intercellular cell adhesions. P-cadherin contains a cytoplasmic carboxy-terminal domain, a transmembrane domain, and a extracellular N-terminal domain. P-cadherin was first identified in mouse placental tissue. However, in humans, the distribution of P-cadherin is restricted to the basal or lower layers of the stratified epithelia in the epithelial layer, and is not found in human placental tissue. In humans, the loss of functional P-cadherin can induce characteristic genetic syndromes such as hypotrichosis with juvenile muscular dystrophy (HJMD) and ectodermal dysplasia, ectrodactyly, and muscular dystrophy (EEM syndrome). In breast cancer, an overexpression of P-cadherin is often found in high histological grade tumors. In an in vitro study, the overexpression of P-cadherin in breast cancer cells in the presence of wild type E-cadherin, promoted cell invasiveness, motility, and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full-length Human P-cadherin peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL.  For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed A-431 cells permeabilized with either methanol or Triton X-100. Neither method was compatible with P-Cadherin staining. This clone was also tested using methanol fixation on A-431 and HaCaT cells. Neither cell line was compatible with P-Cadherin staining.

RRID
AB_2894538 (BioLegend Cat. No. 948401)
AB_2894538 (BioLegend Cat. No. 948402)

Antigen Details

Structure
P-Cadherin is a 829 amino acid protein with a predicted molecular weight of 91 kD.
Distribution

Adhesion protein/Plasma membrane

Function
Intercellular cell adhesion
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References
  1. Ribeiro A. et al. 2010. Oncogene. 29:392-402.
  2. Shimoyama Y. and Hirohashi S. 1991. Cancer Research. 51:2185-2192.
  3. Sun L. et al. 2011. American Journal of Pathology. 179:380-390.
  4. Vieira A. & Paredes J. 2015. Molecular Cancer. 14:178.
Gene ID
1001 View all products for this Gene ID
UniProt
View information about P-Cadherin on UniProt.org
Go To Top Version: 1    Revision Date: 09.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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