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Purified anti-p38 MAPK Phospho (Thr180/Tyr182) Antibody (Previously Covance catalog# PRB-510P)

Pricing & Availability
Clone
Poly29035 (See other available formats)
Regulatory Status
RUO
Other Names
Mitogen-activated protein kinase 14, MAP kinase 14, p38alpha Exip, p38 MAP kinase, MAP kinase Mxi2, MAP kinase p38 alpha, CSAID-binding protein, MAX-interacting protein 2, stress-activated protein kinase 2A, p38 mitogen activated protein kinase
Previously
Covance Catalog# PRB-510P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly29035_Purified_p38-MAPK_Antibody_1_052019
Figure Legend: IHC staining of purified anti-p38 MAPK Phospho (Thr180/Tyr182) antibody (clone Poly29035) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:10 dilution of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • Poly29035_Purified_p38-MAPK_Antibody_1_052019
    Figure Legend: IHC staining of purified anti-p38 MAPK Phospho (Thr180/Tyr182) antibody (clone Poly29035) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:10 dilution of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • Poly29035_Purified_p38-MAPK_Antibody_2_052019
    Total cell lysates (15 µg total protein) from Jurkat cells untreated (-) and treated (+) with anisomycin (25 µg/mL for 15 minutes) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-p38 MAPK Phopsho (Thr180/Tyr182) Antibody, clone Poly29035, at a 1:500 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Purified anti-p38 MAPK Antibody, clone Poly6224 (Cat. No. 622402), and Direct-Blot™ anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:500 dilution. Lane M: Molecular Weight marker.
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903501 100 µL 288 CHF
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Description

The p38 MAP kinase is a stress-activated protein kinase. The p38 MAP kinase is activated by treatment of cells with proinflammatory cytokines (e.g. TNF and IL-1) or by exposure of cells to environmental stress (e.g. UV radiation and osmotic shock). This activation results in the phosphorylation of residues Thr180 and Tyr182. This antibody is specific for the 39 kD p-38 protein phosphorylated at Thr180/Tyr182 It is purified by sequential chromatography on protein A and phospho- and dephospho- peptide affinity columns.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA, and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1 is suggested. For Western blotting, the suggested use of this reagent is 1:1000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Product Citations
  1. Gärtner K, et al. 2018. Cancer Med. 11:39. PubMed
  2. Anerillas C, et al. 2022. Sci Adv. 8:eabm0756. PubMed
  3. Anerillas C, et al. 2022. Nat Commun. 13:6228. PubMed
RRID
AB_2565031 (BioLegend Cat. No. 903501)

Antigen Details

Structure
p38 MAPK is a with a predicted molecular weight of 39 kD
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Phospho-Proteins, Presynaptic proteins
Gene ID
1432 View all products for this Gene ID
UniProt
View information about p38 MAPK Phospho Thr180/Tyr182 on UniProt.org
Go To Top Version: 5    Revision Date: 05.20.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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