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Purified anti-PADI4 Antibody

Pricing & Availability
Clone
O94H5 (See other available formats)
Regulatory Status
RUO
Other Names
Peptidylarginine deiminase 4, Peptidyl Arginine Deiminase, Type IV, PADI5, HL-60 PAD, Protein-Arginine Deiminase Type-4, PAD4
Isotype
Mouse IgG2a
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Product Citations
publications
O94H5_PURE_PADI4_Antibody_1_031919
Whole cell extracts (15 µg protein) from HL-60 cells treated (+) or untreated (-) with 1.25% DMSO for 5 days were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL of Purified anti-PADI4 Antibody (1:500 dilution) Antibody, clone O94H5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
  • O94H5_PURE_PADI4_Antibody_1_031919
    Whole cell extracts (15 µg protein) from HL-60 cells treated (+) or untreated (-) with 1.25% DMSO for 5 days were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL of Purified anti-PADI4 Antibody (1:500 dilution) Antibody, clone O94H5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Check Availability Save
684202 100 µg 288 CHF
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Description

PADI4 is an enzyme that catalyzes peptidyl arginine to citrulline in the presence of Ca2+ ions. This reaction, known as citrullination, leads to the post-translational modification of proteins. PADI4 was initially cloned from the HL-60 cell line (human promyelocytic leukemia cells). It is mainly expressed in granulocytes, monocytes and CD34+ stem cells. PADI4 may play a role in granulocyte and macrophage development leading to inflammation and immune response. The PADI4 single-nucleotide polymorphism (SNP) and haplotypes were associated with rheumatoid arthritis susceptibility. Recently, PADI4 has also been found in a variety of malignant tumors and implies its potential roles in tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein of human PADI4 expressed in HEK293T.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Palko SI 2022. Journal of Neuroscience Research. N/A. PubMed
  2. Kang L, et al. 2020. Nat Commun. 11:2488. PubMed
  3. Vaibhav K, et al. 2020. Sci Adv. 6:eaax8847. PubMed
RRID
AB_2572090 (BioLegend Cat. No. 684202)

Antigen Details

Structure
663 amino acid with the predicted molecular weight of approximately 74kD.
Distribution

PADI4 is mainly expressed in granulocytes, monocytes and CD34+ stem cells.

Function
PADI4 may play a role in granulocyte and macrophage development leading to inflammation and immune response.
Interaction
Histone H1, Histone H3, Histone H4, and SOX4.
Cell Type
Granulocytes, Hematopoietic stem and progenitors, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity
Antigen References

1. Abd-Allah SH, et al. 2012. Joint Bone Spine 79:124.
2. Worthington J, et al. 2003. Trends Mol. Med. 9:405.
3. Song G, et al. 2016. Oncotarget. 7:3144-57.
4. Kolodziej S, et al. 2014. Nat. Commun. 5:3995.
5. Suzuki A, et al. 2003. Nat. Genet. 34:395.
6. Chang X, et al. 2010. Cancer Cell International 10:7.

Gene ID
23569 View all products for this Gene ID
UniProt
View information about PADI4 on UniProt.org
Go To Top Version: 2    Revision Date: 03.19.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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