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Purified anti-PCSK9 Antibody

Pricing & Availability
Clone
M1110F3 (See other available formats)
Regulatory Status
RUO
Other Names
Neural Apoptosis Regulated Convertase 1 (NARC1), proprotein convertase PC9, subtilisin/kexin-like protease PC9, FH3, HCHOLA3, AI415265, AI747682
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
M1110F3_PURE_PCSK9_Antibody_WB_070215
Recombinant mouse PCSK9 (lane 1, 50 ng) and recombinant human PCSK9 (lane 2, 50 ng) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-PCSK9 antibody (clone M1110F3). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • M1110F3_PURE_PCSK9_Antibody_WB_070215
    Recombinant mouse PCSK9 (lane 1, 50 ng) and recombinant human PCSK9 (lane 2, 50 ng) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-PCSK9 antibody (clone M1110F3). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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677502 100 µg 265 CHF
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Description

Proprotein convertase subtilisin/kexin 9 (PCSK9) possesses a signal peptide, a prosegment, a catalytic domain, a hinge region, and a C-terminal Cys-His-rich domain. This protein is primarily synthesized and secreted by hepatocytes. Besides the liver, it is also expressed in the small intestine, kidney, and brain. It is also present in the plasma. Upon translocation to the endoplasmic reticulum, the prosegment of PCSK9 is autocatalytically cleaved and secreted as a stable, enzymatically inactive, non-covalent complex. Elevated low density lipoprotein-cholesterol (LDLc) level is a major risk factor for cardiovascular disease and atherosclerosis. PCSK9 is a regulator of LDLc levels through the binding of LDLR, which subsequently leads to the degradation of LDLR. PCSK9 null mice fed with a high fat diet demonstrated a four-fold decrease in cholesterol accumulation, which stresses the important role played by PCSK9 in the development of cardiovascular disesases. PCS59 also binds to other LDLR family members such as very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER), and apolipoprotein receptor 2 (LRP8/APOER2), which leads to their degradation in the intracellular acidic compartments. Like the LDLR, gene expression of PCSK9 is positively regulated by sterol regulatory element-binding protein 2 (SREBP-2), which is a transcription factor that is activated in response to cellular cholesterol depletion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse PCSK9, amino acids Gln35-Gln694, expressed with a C-terminal His tag and a linker sequence in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sun H, et al. 2018. J Lipid Res. 59:207. PubMed
RRID
AB_2565908 (BioLegend Cat. No. 677502)

Antigen Details

Structure
Monomer. The molecular mass of a full-length and mature protein is 74.8 kD and 53.7 kD, respectively.
Distribution
Hepatocytes, renal cells, neuroepithelioma, and Schwann cells.
Function
PCSK9 regulates LDLR, VLDLR, APOER, and APOER2 levels, and therefore, LDLc levels. PCSK9 is regulated by SREBP-2.
Interaction
Hepatocytes.
Ligand/Receptor
LDLR, VLDLR, APOB, LRP1, and LRP8.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators
Antigen References

1. Seidah NG, et al. 2003. Proc. Natl. Acad. Sci. USA 100:928.
2. Naureckiene S, et al. 2003. Arch. Biochem. Biophys. 420:55.
3. Costet P, et al. 2008. Trends Biochem. Sci. 33:426.
4. Grefhorst A, et. al. 2008. J. Lipid Res. 49:1303.
5. Tavori H, et al. 2013. Circulation 127:2403.
6. Denis M, et al. 2012. Circulation 125:894.
7. Tibolla G, et al. 2011. Nutr. Metab. Cardiovasc. Dis. 21:835.

Gene ID
100102 View all products for this Gene ID
UniProt
View information about PCSK9 on UniProt.org
Go To Top Version: 1    Revision Date: 07.02.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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