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Purified anti-PER1 Antibody

Pricing & Availability
Clone
W17208B (See other available formats)
Regulatory Status
RUO
Other Names
Period Circadian Regulator 1, Period Circadian Protein Homolog 1, Circadian Clock Protein PERIOD 1, Period Circadian Clock 1, RIGUI, HPER1, PER, Circadian Pacemaker Protein RIGUI, Circadian Pacemaker Protein Rigui
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17208B_PURE_PER1_Antibody_1_031120_updated.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-PER1 antibody (clone W17208B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W17208B_PURE_PER1_Antibody_1_031120_updated.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-PER1 antibody (clone W17208B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W17208B_PURE_PER1_Antibody_2_031120_updated.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-PER1 antibody (clone W17208B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a mouse control antibody against a separate epitope of PER1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker.
  • W17208B_PURE_PER1_Antibody_3_031120_updated.png
    Whole cell extracts (15 µg protein) from HeLa cells transfected with scramble control siRNA or siRNA targeting PER1 were resolved on a 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 0.1 µg/mL of purified anti-PER1 antibody (clone W17208B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. siRNA targeting PER1 for depletion was obtained from Dharamcon (SMARTpool ON-TARGETplus PER1 siRNA, Cat. No. L-011350-00-0005)
Cat # Size Price Quantity Check Availability Save
936001 25 µg 101 CHF
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936002 100 µg 253 CHF
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Description

PER1 (Period Circadian Regulator 1), a transcriptional repressor, is a component of the circadian clock and a member of the Period family. The circadian clock is the body’s internal time keeping mechanism, which regulates various physiological processes including hormone rhythms, locomotor activity, sleep cycle, and behavior. PER1 is expressed throughout peripheral tissues but expression is most significant in the suprachiasmatic nucleus (SCN) region of the brain which functions as the primary circadian pacemaker in mammals. PER1 is expressed in an oscillating rhythm with a period of about 24 hours which is maintained even in constant darkness. PER1 expression is light dependent, with exposure to light during subjective night increasing expression and shifting its phase in the SCN. Full length PER1 localizes to the cytoplasm with a shorter nuclear isoform localizing in the nucleus. Diseases associated with PER1 include periodic limb movement disorder (PLMD), advanced sleep phase syndrome, cardiovascular diseases and cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PER1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone recognizes human and mouse PER1.

Clone is not recommended for ICC.

Product Citations
  1. Tampakakis E, et al. 2021. Sci Adv. 7:eabh4181. PubMed
RRID
AB_2832904 (BioLegend Cat. No. 936001)
AB_2832904 (BioLegend Cat. No. 936002)

Antigen Details

Structure
PER1 is a 1290 amino acid protein with a predicted molecular weight of 136 kD.
Distribution

PER1 is mainly localized to the nucleus, with additional localization to the cytosol.

Function
Component of the circadian clock
Interaction
TIMELESS, PER2, PER3, CRY1 and CRY2
Ligand/Receptor
NR1D1/2 and RORA/B/G
Cell Type
Neurons
Biology Area
Neuroscience
Antigen References
  1. Gery S, et al. 2006. Mol Cell. 22:3:375.
  2. Chen S, et al. 2005. Carcinogenesis. 26:7:1241.
  3. Yan L, et al. 1999. Neuroscience. 94:1:141.
  4. Shigeyoshi Y, et al. 1997. Cell. 91:7:1043.
Gene ID
5187 View all products for this Gene ID
UniProt
View information about PER1 on UniProt.org
Go To Top Version: 1    Revision Date: 03.09.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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