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Purified anti-PGK1 Antibody

Pricing & Availability
Clone
W18295A (See other available formats)
Regulatory Status
RUO
Other Names
Phosphoglycerate Kinase 1 (PGK1), cell migration-inducing gene 10 protein, primer recognition protein 2 (PRP2), Epididymis Secretory Sperm Binding Protein Li 68p, EC 2.7.2.3
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W18295A_PURE_PGK1_Antibody_1_030822
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-PGK1 (clone W18295A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • W18295A_PURE_PGK1_Antibody_1_030822
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-PGK1 (clone W18295A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • W18295A_PURE_PGK1_Antibody_2_030822
    IHC staining of purified anti-PGK1 (clone W18295A) on formalin-fixed paraffin-embedded mouse eye tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1x (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the product’s protocol. The image was captured with a 40X objective. Scale bar: 50 µm
  • W18295A_PURE_PGK1_Antibody_3_030822
    ICC staining of purified anti-PGK1 (clone W18295A) on HeLa cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with either purified rat IgG2a, λ isotype control (Cat. No. 402301) (left panel) or purified anti-PGK1 (clone W18295A) (right panel) at 1.0 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) (Cat. No. 422801), and the image was captured with a 40X objective. Scale bar = 50 µm
Cat # Size Price Quantity Check Availability Save
603251 25 µg 118 CHF
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603252 100 µg 293 CHF
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Description

Phosphoglycerate kinase 1 (PGK1) is one of two enzymes, along with its isozyme PGK2, which catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, and transfers the phosphate group to ADP to produce ATP. This step is notable as the “break even” point of glycolysis where ATP expenditure matches ATP output. Additionally, PGK1 functions as a protein kinase, and is also involved in transcription initiation as a cofactor for DNA polymerase alpha. The gene encoding PGK1 is on the X chromosome, and mutations in PGK1 are associated with various cancers, and X-linked recessive variants of anemia, mental disorders, and myopathy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial fragment of recombinant Human PGK1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910481 (BioLegend Cat. No. 603251)
AB_2910481 (BioLegend Cat. No. 603252)

Antigen Details

Structure
PGK1 is a 417 amino acid protein with a predicted molecular weight of 44.6 kD.
Distribution

PGK1 is widely expressed in the cytoplasm of most tissues.

Function
Enzyme catalyzing the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Protein kinase.
Interaction
PDHK1
Biology Area
Cancer Biomarkers
Molecular Family
Enzymes and Regulators, Protein Kinases/Phosphatase
Antigen References
  1. Fu Q and Yu Z. 2020. Life Sci. 256:117863.
Gene ID
5230 View all products for this Gene ID
UniProt
View information about PGK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.08.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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