- Clone
- W18205A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein Kinase C iota, Atypical Protein Kinase C-lambda/iota, Protein Kinase C iota Type, PRKC-lambda/iota, aPKC-lambda/iota, atypical protein kinase C, aPKC, aPKCλ/ι
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18205A_PURE_PKCi_Antibody_1_103019 W18205A_PURE_PKCi_Antibody_1_103019](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18205A_PURE_PKCi_Antibody_1_103019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PKCι antibody (clone W18205A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of ascending PRKCI expression; predicted expression data obtained from Human Protein Atlas. -
Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400602) or purified anti-PKCι antibody (clone W18205A). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of clone W18205A. The secondary step was performed using light-chain-specific secondary antibodies. Lane M: Molecular weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
671201 | 25 µg | 101 CHF | ||||
671202 | 100 µg | 253 CHF |
Members of the protein kinase c family (PKC) are serine/threonine protein kinases with a broad set of substrates that can regulate diverse cellular processes. PKCι, also referred to as PKCι/λ, is a member of the atypical subfamily of PKC, which is characterized by complete independence of calcium and diacylglycerol as second messengers. It has been shown to play a role in cancer survival through multiple mechanisms: a. phosphorylation of the DNA-binding domain of FoxO1 resulting in transcriptional reprogramming of c-myc, which leads to increased proliferation of angiosarcoma cells, b. regulating membrane trafficking of type 1-matrix metalloprotease from late endosomal compartments to the plasma membrane, and c. mediating pro-survival by phosphorylating and inhibiting the pro-apoptotic factor BAD in glioblastomas. PKCι is also required for resistance to anti-apoptotic drugs in BCR-Abl-dependent leukemia and may represent a viable target for combinatorial therapies.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human PKCι protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with methanol or Triton X-100; it failed due to weak staining.
- RRID
-
AB_2820114 (BioLegend Cat. No. 671201)
AB_2820114 (BioLegend Cat. No. 671202)
Antigen Details
- Structure
- PKCι is a 596 amino acid protein with a predicted molecular weight of 68 kD.
- Distribution
-
Cytosol and Nucleus/Ubiquitously expressed
- Function
- Protein Kinase/Cell Signaling
- Biology Area
- Cell Biology
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Nayak RC, et al. 2019. Nat. Commun. 10:46.
- Riddell M, et al. 2018. Nat. Commun. 9:5357.
- Rosse C, et al. 2014. Proc Natl Acad Sci U S A. 111:E1872.
- Desai S, et al. 2011. Biochim Biophys Acta. 1813:1190.
- Ishiguro H, et al. 2009. Proc Natl Acad Sci U S A. 106:16369.
- Gene ID
- 5584 View all products for this Gene ID
- UniProt
- View information about PKCiota on UniProt.org
Related FAQs
Other Formats
View All PKCι Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PKCι | W18205A | WB,IP |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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