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Purified anti-PLAP Antibody

Pricing & Availability
Clone
8A11-C11-C9 (See other available formats)
Regulatory Status
RUO
Other Names
ALPP, PLAA, , Placental Alkaline Phosphatase 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8A11-C11-C9_PURE_PLAP_Antibody_1_062619
IHC staining of purified anti-PLAP antibody (clone 8A11-C11-C9) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8A11-C11-C9_PURE_PLAP_Antibody_1_062619
    IHC staining of purified anti-PLAP antibody (clone 8A11-C11-C9) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8A11-C11-C9_PURE_PLAP_Antibody_2_062619
    Western blot of purified anti-PLAP antibody (clone 8A11-C11-C9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human placenta lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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866901 25 µg 106 CHF
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866902 100 µg 276 CHF
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Description

PLAP is a membrane-bound glycosylated dimeric enzyme that was initially identified in the placenta. PLAP is also known as phospholipase A2 activating protein. Abnormal amyloid beta-induced activation of synaptic phospholipase A2 results in synaptic degeneration due to elevated levels of platelet-activating factor, suggesting that PLAP may be a contributing factor in synaptic damage observed in Alzheimer’s disease patients. Clinically, PLAP is broadly used as a tumor marker, especially in geminoma, seminoma, and ovarian cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810793 (BioLegend Cat. No. 866901)
AB_2810793 (BioLegend Cat. No. 866902)

Antigen Details

Structure
PLAP is a 795 amino acid protein with predicted molecular mass of ~87 kD and observed molecular mass of ~70 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane

Function
Phospholipase A2 activating protein that may be involved in amyloid beta-induced synaptic degeneration.
Interaction
Ubiquitin, UBXN6, VCP, YOD1
Cell Type
Astrocytes, Endothelial cells, Neurons
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Watanabe S et al., 2012. Pediatr Neurosurg. 48(3):141
  2. Bate C et al., 2010. Mol Neurodegener. 5:13
  3. Huynh ML et al., 2011. Genesis. 49(11):851
Gene ID
18786 View all products for this Gene ID
UniProt
View information about PLAP on UniProt.org
Go To Top Version: 2    Revision Date: 07.15.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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