Purified anti-PME-1 Antibody (Previously Covance catalog# MMS-5239)

Pricing & Availability
Clone
8A6-F8 (See other available formats)
Regulatory Status
RUO
Other Names
Protein phosphatase methylesterase 1, protein phosphatase methylesterase-1, PME1
Previously
Covance Catalog# MMS-5239
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_8A6-F8_Purified_PME-1_Antibody_0_WB_050218
Western blot of purified anti-PME-1 antibody (clone 8A6-F8) (upper). Lane 1: 15 µg of 293T cell lysate; Lane 2: 15 µg 293T/PME-1 CRISPR/Cas9 knockout (KO) cell lysate; Lane M: Molecular weight marker. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Enhanced chemiluminescence was used as the detection system.
  • 1_8A6-F8_Purified_PME-1_Antibody_0_WB_050218
    Western blot of purified anti-PME-1 antibody (clone 8A6-F8) (upper). Lane 1: 15 µg of 293T cell lysate; Lane 2: 15 µg 293T/PME-1 CRISPR/Cas9 knockout (KO) cell lysate; Lane M: Molecular weight marker. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Enhanced chemiluminescence was used as the detection system.
  • 8A6-F8_PUREAF_PME-1_Antibody_2_120518.png
    IHC staining of purified anti-PME-1 antibody (clone 8A6-F8) on formalin-fixed paraffin-embedded mouse pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8A6-F8_PUREAF_PME-1_Antibody_3_120518.png
    IHC staining of purified anti-PME-1 antibody (clone 8A6-F8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8A6-F8_PUREAF_PME-1_Antibody_4_120518.png
    IHC staining of purified anti-PME-1 antibody (clone 8A6-F8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8A6-F8_PUREAF_PME-1_Antibody_5_120518.png
    Western blot of purified anti-PME-1 antibody (clone 8A6-F8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
820901 100 µL 275 CHF
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Description

Protein phosphatase methylesterase-1 (PME-1) is an enzyme that catalyzes the demethylation and inactivation of protein phosphatase (PP2A). PP2A is a heterotrimeric serine/threonine phosphatase that regulates proteins of oncogenic signaling pathways such as Raf, MEK and Akt. PP2A is associated with growth inhibition and cell cycle arrest.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a HIS-tagged, full-length mouse PME1 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P, KO/KD-WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P).

RRID
AB_2564845 (BioLegend Cat. No. 820901)

Antigen Details

Structure
Expected MW: 42 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Protein Kinases/Phosphatase
Gene ID
51400 View all products for this Gene ID
UniProt
View information about PME-1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All PME-1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-PME-1 8A6-F8 WB,IHC-P,KO/KD-WB
Go To Top Version: 5    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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