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Purified anti-Podoplanin (Lymphatic Endothelial Marker) Antibody

Pricing & Availability
Clone
D2-40 (See other available formats)
Regulatory Status
RUO
Other Names
Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_D2-40_PURE_Podoplanin_Antibody_3_IHC_012618
IHC staining of purified anti-Podoplanin (Lymphatic Endothelial Marker) antibody (clone D2-40) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 1.3 µg/ml of the primary antibody at room temperature for 60 minutes. BioLegend's Ultra-Streptavidin (USA) HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 1_D2-40_PURE_Podoplanin_Antibody_3_IHC_012618
    IHC staining of purified anti-Podoplanin (Lymphatic Endothelial Marker) antibody (clone D2-40) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 1.3 µg/ml of the primary antibody at room temperature for 60 minutes. BioLegend's Ultra-Streptavidin (USA) HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 2_D2-40_PURE_Podoplanin_Antibody_2_IHC_060215
    IHC staining of purified anti-Podoplanin (Lymphatic Endothelial Marker)antibody (clone D2-40) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 3_D2-40_PURE_Podoplanin_Antibody_1_WB_111417
    Western blot analysis of cell lysates from HeLa using Podoplanin Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • 4_D2-40_PURE_Podoplanin_Antibody_4_ICFC_032724
    LN319 cells (positive control, filled histogram), or HeLa cells (negative control, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and stained with Purified anti-Podoplanin (clone D2-40), or Purified mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 401401), followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322)
  • 5_D2-40_PURE_Podoplanin_Antibody_5_ICC_032724
    HeLa cells (negative control, top panels), or LN319 cells (positive control, bottom panels) were fixed and permeabilized with 2% PFA and ice-cold methanol, and then intracellularly stained with Purified anti-Podoplanin (clone D2-40) (right panels) or Purified mouse IgG1, κ isotype control (Cat. No. 401401) (left panels). Nuclei were counterstained with DAPI (Cat. No. 422801) Scale bar 50 µm
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916605 25 µg 124 CHF
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916606 100 µg 265 CHF
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Description

Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
M2A protein derived from human germ cell tumors.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB, ICFC, ICC - Verified
ELISA Detection - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/mL is suggested. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg per mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA Detection5 and immunocytochemistry4.

Application References

(PubMed link indicates BioLegend citation)
  1. Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC-P)
  2. Choi WW, et al. 2005. Mod. Pathol. 18:143. (IHC-P)
  3. Chu AY, et al. 2005. Mod. Pathol. 18:105. (IHC-P) PubMed
  4. Yoon C, et al. 2008. Blood 112:1129. (ICC) PubMed
  5. Schacht V, et al. 2005. American Journal of Pathology 166:913. (ELISA Detection, WB) PubMed
  6. Marks A, et al. 1999. British Journal of Cancer 80:569.
Product Citations
  1. Burlingame EA, et al. 2021. Cell Rep Methods. 1:. PubMed
  2. Yoon C, et al. 2008. Blood. 112:1129-1138. PubMed
  3. Francescone R, et al. 2020. Cancer Discovery. 11(2):446-479. PubMed
  4. Schacht V, et al. 2005. Am J Pathol. 166:913-921. PubMed
  5. Chu A, et al. 2005. Mod Pathol. 18:105-110. PubMed
RRID
AB_2565820 (BioLegend Cat. No. 916605)
AB_2565820 (BioLegend Cat. No. 916606)

Antigen Details

Structure
35 kD.
Distribution

Membrane and plasma membrane.

Function
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
Interaction
Cytoskeletal signaling.
Biology Area
Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org
Go To Top Version: 4    Revision Date: 03.27.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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