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Purified anti-POU2AF1 (OBF1) Antibody

Pricing & Availability
Clone
2E9C01 (See other available formats)
Regulatory Status
RUO
Other Names
POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1 (OBF1), BOB-1, OCA-B, OCT-binding factor 1
Isotype
Mouse IgG1, κ
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Product Citations
publications
2E9C01_PURE_POU2AF1_Antibody_WB_2_122017
Total lysates (15 µg protein) from Daudi (Human, Positive control), Jurkat (Human, Negative control) and A20 (Mouse) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/ml) purified anti-POU2AF1 antibody, clone 2E9C01. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 2E9C01_PURE_POU2AF1_Antibody_WB_2_122017
    Total lysates (15 µg protein) from Daudi (Human, Positive control), Jurkat (Human, Negative control) and A20 (Mouse) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/ml) purified anti-POU2AF1 antibody, clone 2E9C01. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
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682502 100 µg 347 CHF
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Description

POU2AF1, also known as OBF1, is a B lymphocyte specific transcriptional coactivator. POU2AF1 plays important regulatory roles in the function and maturation of B cells. It stimulates transcription of the immunoglobulin genes by interaction with Oct-1 or Oct-2. The POU2AF1 gene encodes a single mRNA that produces two isoforms, p34 and p35, by post-translational processing. Both isoforms are expressed at similar levels and are able to stimulate octamer-dependent transcription. The cytoplasmic p35 isoform of POU2AF1 regulates pre-B cell proliferation and BCR signaling through interacting with the tyrosine kinase Syk. POU2AF1 deficient mice have shown reduced numbers of circulating B cells in the bone marrow and failed to form germinal centers in the spleen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human POU2AF1 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The POU2AF1 gene encodes a single mRNA that produces two isoforms, p34 and p35. This antibody recognizes both.

RRID
AB_2566625 (BioLegend Cat. No. 682502)

Antigen Details

Structure
256 amino acids with a predicted molecular weight of 27.4 kD.
Distribution

Nucleus.

Function
POU2AF1 is a B-cell specific transcription factor that participates in the regulation of T cell dependent humoral immune response and formation of the germinal center in the spleen.
Interaction
POU2AF1 interacts with OCT1, OCT2, SIAH1, SIAH2, and SYK.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Corcoran L, et al. 2014. Front Immunol. 5:108.
2. Bordon A, et al. 2008. PLoS One 3:e4007.
3. Zhao C, et al. 2008. Oncogene. 27:63.
4. Lins K, et al. 2003. EMBO. J. 22:2188.
5. Nielsen PJ, et al. 1996. Eur. J. Immunol. 26:3214.
6. Francesconi SC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:4089.

Gene ID
5450 View all products for this Gene ID
UniProt
View information about POU2AF1 on UniProt.org
Go To Top Version: 1    Revision Date: 12.02.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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