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- Clone
- 2A10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, PPP2CA
- Previously
-
Covance Catalog# MMS-5117
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
N-terminally HA-tagged PPH21 was immunoprecipitated with 12CA5 anti-HA antibody (cross linked to protein A sepharose) from lysates of wtBY4741 (left) or PPM1δ (right) (lacking the PP2A methyltransferase) yeast cells, separated by 10% SDS-PAGE and blotted onto nitrocellulose membrane. Blots were incubated at 4°C o/n in a Miniblotter 45 (Immunetics) with 2A10 at the indicated dilutions. Anti-HA tag clone 16B12 served as positive control.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 828801 | 100 µL | 335 CHF | ||||
PP2AC (Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform), encoded by the PPP2CA gene, is the main phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2AC is reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). This carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, which in turn, modulates the PP2A holoenzyme's enzyme activity and cellular localization.
Product DetailsProduct Details
- Verified Reactivity
- Yeast
- Reported Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide from Human methylated Leucine 309 PP2A catalytic subunit.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100 - 1:800*
- Application Notes
-
This antibody is effective in immunoblotting (WB).
* Expected MW: 36 kD
WB Positive Control: BY4741 yeast cells
The immunogen sequence (C-ßA-RRTPDYFL-Ome) is conserved from human, mouse to yeast. - Product Citations
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- RRID
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AB_2564923 (BioLegend Cat. No. 828801)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Gene ID
- 5515 View all products for this Gene ID
- UniProt
- View information about PP2AC Methyl Leu309 on UniProt.org
Related FAQs
Other Formats
View All PP2AC Methyl (Leu309) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-PP2AC Methyl (Leu 309) | 2A10 | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-PP2AC Methyl (Leu 309)
N-terminally HA-tagged PPH21 was immunoprecipitated with 12C...
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