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Purified anti-PPP3CA (CnA1) Antibody (Previously Covance catalog# MMS-5123)

Pricing & Availability
Clone
2E2.D11 (See other available formats)
Regulatory Status
RUO
Other Names
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, calcineurin A alpha, CAM-PRP catalytic subunit, calmodulin-dependent calcineurin A subunit alpha isoform, protein phosphatase 2B, protein phosphatase 3
Previously
Covance Catalog# MMS-5123
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_2E2-D11_PURE_PPP3CA_3_010220.png
Western blot of purified anti-PPP3CA (CnA1) antibody (clone 2E2.D11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_2E2-D11_PURE_PPP3CA_3_010220.png
    Western blot of purified anti-PPP3CA (CnA1) antibody (clone 2E2.D11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_2E2dotD11_Purified_PPP3CA_Antibody_1_020619_updated.png
    IHC staining of purified anti-PPP3CA (CnA1) antibody (clone 2E2.D11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_2E2dotD11_Purified_PPP3CA_Antibody_2_020619_updated.png
    IHC staining of purified anti-PPP3CA (CnA1) antibody (clone 2E2.D11) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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817601 100 µL 275 CHF
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Description

PPP3CA (Protein Phosphatase 3, Catalytic Subunit, Alpha Isozyme) is also known by a handful of other names, including CALNA, PPP2B, Calcineurin A Alpha, and CNA1. It is one of the subunits of the heterodimer that forms calcineurin. Calcineurin functions as a calcium-dependent, calmodulin-stimulated protein phosphatase that activates T cells via dephosphorylation of the transcription factor NFATc.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence conjugated to KLH corresponding to amino acids 478-492 of human CnA1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1 µg/mL. For immunohistochemistry, a concentration range of 1 - 5 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564812 (BioLegend Cat. No. 817601)

Antigen Details

Structure
Expected MW: 60 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
5530 View all products for this Gene ID
UniProt
View information about PPP3CA on UniProt.org
Go To Top Version: 5    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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