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Purified anti-Presenilin 1 (N terminus) Antibody (Previously Covance catalog# SIG-39194)

Pricing & Availability
Clone
NT1 (See other available formats)
Regulatory Status
RUO
Other Names
AD3, FAD, PS-1, S182, presenilin-1, gamma-secretase
Previously
Covance Catalog# SIG-39194
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
NT1_Purified_Presenilin-1_Antibody_1_072318
Western blot of purified anti-Presenilin 1 (N terminus) antibody (clone NT1). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain cerebellum membrane lysate; Lane 3: 40 µg of human brain cortex membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NT1_Purified_Presenilin-1_Antibody_1_072318
    Western blot of purified anti-Presenilin 1 (N terminus) antibody (clone NT1). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain cerebellum membrane lysate; Lane 3: 40 µg of human brain cortex membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NT1_Purified_Presenilin-1_Antibody_2_072318
    Western blot of purified anti-Presenilin 1 (N terminus) antibody (clone NT1). Lane 1: Molecular weight marker; Lane 2: 40 µg of HeLa lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
823404 25 µL 112 CHF
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823401 200 µL 501 CHF
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Description

Presenilin 1 is one of two transmembrane related-proteins, the other being Presenilin 2, that form the catalytic subunits of the gamma-secretase intramembrane protease complex. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein (APP), Notch, Erb4 and E-cadherin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BioLegend's mouse anti-Presenilin 1 was raised against a portion of the n-terminal region of Presenilin 1 (RRSLGHPEP).
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is against the N-terminus of Presenilin 1 and detects the N-terminal fragment (NTF) generated from the proteolysis of the full-length protein (52kD).

Application References

(PubMed link indicates BioLegend citation)
  1. Mathews PM, et al. 2000. Mol Med. 6(10)
  2. Cohen RM, et al. 2013. J. Neurosci. 15:6245. (WB) PubMed
  3. Laudon H, et al. 2004. J Neurochem. 89:44-53
  4. Ahn K, et al. 2010. Proc Natl Acad Sci U S A. 107:21435
Product Citations
  1. Bohl B, et al. 2022. Sci Adv. 8:eabl5792. PubMed
  2. Cohen R, et al. 2013. J Neurosci. 33:6245-6256. PubMed
RRID
AB_2564868 (BioLegend Cat. No. 823404)
AB_2564868 (BioLegend Cat. No. 823401)

Antigen Details

Structure
Expected MW: 26 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Secretases
Gene ID
5663 View all products for this Gene ID
UniProt
View information about Presenilin 1 NH2 terminus on UniProt.org
Go To Top Version: 5    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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