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Purified anti-PSMD9 Antibody

Pricing & Availability
Clone
Poly28615 (See other available formats)
Regulatory Status
RUO
Other Names
26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27, Proteasome 26S Subunit, Non-ATPase 9, Homolog of Rat Bridge 1, Rpn4, P27
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28615_Purified_PSMD9_Antibody_1_051619.png
Western blot of purified anti-PSMD9 antibody (clone Poly28615). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28615_Purified_PSMD9_Antibody_1_051619.png
    Western blot of purified anti-PSMD9 antibody (clone Poly28615). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28615_Purified_PSMD9_Antibody_2_051619.png
    Western blot of purified anti-PSMD9 antibody (clone Poly28615). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28615_Purified_PSMD9_Antibody_3_051619.png
    IHC staining of purified anti-PSMD9 antibody (clone Poly28615) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28615_Purified_PSMD9_Antibody_4_051619.png
    IHC staining of purified anti-PSMD9 antibody (clone Poly28615) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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861501 25 µg 125 CHF
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Description

The ubiquitin proteasome system (UPS) is one of the major protein degradation and clearance pathways in eukaryotic cells. Defects in the UPS system lead to a variety of diseases. The 26S proteasome is composed of a proteolytically active 20S proteasome core particle (CP) and a 19S proteasome regulatory particle (RP). The RP is responsible for substrate binding, unfolding, translocation to the proteolytical chamber of CP and release of ubiquitin from the substrate.

PSMD9, also known as p27, is a subunit of the modulator trimer complex that facilitates the association of PA700 regulator with the 20S proteasome CP to form the ATP-dependent active 26S proteasome. PSMD9 is ubiquitously expressed and is particularly abundant in the liver and kidney. PSMD9 has been implicated in variety of diseases including neurodegeneration, cancer, diabetes, and mental health disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Drosophila
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant PSMD9 (Ser2-Arg223)
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5 µg per ml for human and mouse and 5.0 - 10 µg per ml for Drosophila. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810745 (BioLegend Cat. No. 861501)

Antigen Details

Structure
Human PSMD9 is a 223 amino acid protein with a molecular mass of 25 kD.
Distribution

Tissue distribution: Ubiquitously expressed and highly abundant in liver and kidney
Cellular distribution: Cytosol and Nucleus

Function
PSMD9 is a chaperone protein involved in the assembly of 26S proteasome.
Interaction
Interacts with PSMC6, PSMC3, hnRNPA1
Cell Targets
Polyubiquitinated proteins
Cell Type
Neurons
Biology Area
Cell Biology, Protein Misfolding and Aggregation, Synaptic Biology, Ubiquitin/Protein Degradation
Molecular Family
Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References

Watanabe TK, et al. 1998. Genomics. 50(2):241-50.
Funakoshi M,.et al. 2009. Cell. 18(1): 137(5):887-99.
Stanojevic V, et al. 2005. Mol Cell Endocrinol. 237(1-2):67-74

Gene ID
5715 View all products for this Gene ID
UniProt
View information about PSMD9 on UniProt.org
Go To Top Version: 2    Revision Date: 06.14.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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