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Purified anti-RAGE Antibody

Pricing & Availability
Clone
A17158D (See other available formats)
Regulatory Status
RUO
Other Names
RAGE, AGER, Advanced glycosylation end product-specific receptor
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17158D_PURE_RAGE_Antibody_1_101118
Western blot of purified anti-RAGE antibody (clone A17158D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human lung lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17158D_PURE_RAGE_Antibody_1_101118
    Western blot of purified anti-RAGE antibody (clone A17158D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human lung lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17158D_PURE_RAGE_Antibody_2_101118
    IHC staining of purified anti-RAGE antibody (clone A17158D) on formalin-fixed paraffin-embedded human lung tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Biolegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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855901 25 µg 106 CHF
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855902 100 µg 276 CHF
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Description

RAGE (receptor for advanced glycation end products), is a member of the immunoglobulin super family of proteins with a molecular weight estimated at ~35 kD. RAGE is a pattern recognition receptor as it recognizes a common structure of a class of ligands, including HMGB1, S10012, S100B, S100A7, and amyloid precursor protein (APP).  RAGE may play a role in the pathogenesis of neurodegenerative disorders such as Alzheimer’s disease (AD). Increased RAGE expression levels have been observed in neurons and microglia surrounding senile plaques. In addition, RAGE can mediate the transport of amyloid beta across the blood-brain barrier and its accumulation in the brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human RAGE
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783446 (BioLegend Cat. No. 855901)
AB_2783446 (BioLegend Cat. No. 855902)

Antigen Details

Structure
RAGE is a 404 amino acid protein with an apparent molecular mass of ~43 kD.
Distribution

Tissue Distribution: Lung (major) and colon (less expressed)

Cellular Distribution: Plasma membrane, extracellular, and nucleus

Function
Functions as a pattern recognition receptor in the innate immune system that initiates proinflammatory responses.
Interaction
RAGE forms homo-dimers, and interacts with HMGB1, S100A1, APP, S100B, S100A12, and S100A14.
Ligand/Receptor
AGEs (advanced glycation end-products)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References
  1. Barbezier N, et al. 2014. Ann. Biol. Clin. (Paris) 72:669.
  2. Kalea AZ, et al. 2009. Clin. Sci. (Lond) 116:621.
Gene ID
177 View all products for this Gene ID
UniProt
View information about RAGE on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.12.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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