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Purified anti-rat CD11b/c Antibody

Pricing & Availability
Clone
OX-42 (See other available formats)
Regulatory Status
RUO
Other Names
Rat C3bi receptor, Rat CR3 complement receptor, ITGAM, ITGAX
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
OX-42_Purified_CD11b-c_Antibody_050219_updated.png
LOU rat splenocytes were stained with APC anti-rat CD161 and anti-rat CD11b/c (clone OX-42) Purified (left) or Mouse IgG2a, κ isotype control (right) followed by FITC anti-mouse IgG.
  • OX-42_Purified_CD11b-c_Antibody_050219_updated.png
    LOU rat splenocytes were stained with APC anti-rat CD161 and anti-rat CD11b/c (clone OX-42) Purified (left) or Mouse IgG2a, κ isotype control (right) followed by FITC anti-mouse IgG.
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201801 100 µg 124 CHF
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Description

The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, and NK cells. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat peritoneal macrophages.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of C3bi binding3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC)
  3. Robinson AP, et al. 1986. Immunology 57:239. (Block)
  4. Issekutz SE, et al. 1992. Immunology 76:655. (Block)
  5. Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
  6. Thomas RA. et al. 2009. J Pharmacol Toxicol Methods. 60:263. PubMed
  7. Takeda Y, et al. 2011. Immunobiology. 216:1094. PubMed
  8. Furuhashi K, et al. 2013. J Am Soc Nephrol. PubMed
  9. Cheng HY, et al. 2013. Cytotherapy. 1465:608. PubMed
Product Citations
  1. Ibrahim A, et al. 2011. Diabetes. 60:1122. PubMed
  2. Harms AS, et al. 2017. Acta Neuropathol Commun. 0.267361111. PubMed
  3. Pal S, et al. 2020. Am J Physiol Regul Integr Comp Physiol. R590:318. PubMed
  4. Beetz O, et al. 2019. PLoS One. 14:e0220546. PubMed
RRID
AB_313989 (BioLegend Cat. No. 201801)

Antigen Details

Structure
Integrin family member
Distribution

Monocytes, granulocytes, macrophages, dendritic cells

Function
Receptor for C3bi
Ligand/Receptor
Complement C3bi
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Complement, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Robinson AP, et al. 1986. Immunology 57:239.
2. Barcaly AN. 1981 Immunology 42:593.

Gene ID
25021 View all products for this Gene ID 499271 View all products for this Gene ID
UniProt
View information about CD11b CD11c on UniProt.org
Go To Top Version: 2    Revision Date: 05.02.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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