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Purified anti-RhoG Antibody (Previously Covance catalog# MMS-5032)

Pricing & Availability
Clone
1F3 B3 E5 (See other available formats)
Regulatory Status
RUO
Other Names
ARHG, rho-related GTP-binding protein RhoG, ras homolog gene family, member G (rho G), Rho-G, Rho G
Previously
Covance Catalog# MMS-5032
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1F3-B3-E5_Purified_RhoG_Antibody_1_123118
Western blot of purified anti-RhoG antibody (clone 1F3 B3 E5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain frontal cortex lysate; Lane 3: 20 µg of human brain cerebellum lysate; Lane 4: 20 µg of mouse brain lysate; Lane 5: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1F3-B3-E5_Purified_RhoG_Antibody_1_123118
    Western blot of purified anti-RhoG antibody (clone 1F3 B3 E5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain frontal cortex lysate; Lane 3: 20 µg of human brain cerebellum lysate; Lane 4: 20 µg of mouse brain lysate; Lane 5: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1F3-B3-E5_Purified_RhoG_Antibody_2_123118
    IHC staining of purified anti-RhoG antibody (clone 1F3 B3 E5) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1F3-B3-E5_Purified_RhoG_Antibody_3_123118
    IHC staining of purified anti-RhoG antibody (clone 1F3 B3 E5) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1F3-B3-E5_Purified_RhoG_Antibody_4_123118
    IHC staining of purified anti-RhoG antibody (clone 1F3 B3 E5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
814701 100 µL 275 CHF
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Description

RhoG (Ras homology Growth-related) is also known as ARGH, belonging to the Rho family of small G proteins capable of signaling via binding GTP and catalyzing it to GDP. It is expressed widely in mammalian cells, including fibroblasts, leukocytes, neuronal cells, endothelial cells, and on cell lines, such as HeLa cells. RhoG is involved in a number of signaling pathways, including those for chemokine receptors in cell motility, gene transcription, endocytosis, neurite outgrowth, and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide corresponding to amino acids 161-180 of the RhoG protein conjugated to KLH.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 4.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Meller J, et al. 2008. J Cell Science 121:1981-1989. (WB)
RRID
AB_2564786 (BioLegend Cat. No. 814701)

Antigen Details

Structure
Expected MW: 18 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
391 View all products for this Gene ID
UniProt
View information about RhoG on UniProt.org
Go To Top Version: 4    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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