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Purified anti-RORγ Antibody

Pricing & Availability
Clone
4F3-3C8-2B7 (See other available formats)
Regulatory Status
RUO
Other Names
RORc, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-4F3-3C8-2B7_Pure_RORy_Antibody_031919
Total cell lysates (15 µg total protein) from A549, HepG2 (negative controls) and SR (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-RORγ Antibody, clone 4F3-3C8-2B7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • a-4F3-3C8-2B7_Pure_RORy_Antibody_031919
    Total cell lysates (15 µg total protein) from A549, HepG2 (negative controls) and SR (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-RORγ Antibody, clone 4F3-3C8-2B7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • b-WB_4F3-3C8-2B7_060909
    Nuclerar extract from mouse thymus was resolved by electrophoresis, transferred to nitrocellulose and probed with purified 4F3-3C8-2B7. Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescent system.
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644902 100 µg 293 CHF
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Description

RORγ (Retinoid-related orphan receptor gamma) belongs to the nuclear hormone receptor family, NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. RORγ has two isoforms: γ1 and γ2 (also referred to as RORγt). The RORγt differs from the RORγ1 isoform in that it lacks the amino terminus of RORγ1. RORγ1 contains 516 amino acids and RORγt contains 495 amino acids. RORγ1 has a molecular weight of approximately 58 kD. RORγ1 is highly expressed in thymus, kidney, liver, muscle, and brown fat but not in white fat tissue. RORγt is specifically expressed in only two cell populations, DP thymocytes and lymphoid tissue inducers (LTi). RORγ plays a critical role in control apoptosis during thymopoiesis and T cell homeostasis. RORγ is to regulate TCRα repertoire by virtue of its positive regulatory role on Bcl-x expression. RORγ is essential for lymph nodes and Peyers patches development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human RORγ-GST recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution: 1 to 4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product weakly reacts with Mouse.

Application References

(PubMed link indicates BioLegend citation)
  1. Klemann C, et al. 2009. Am J Pathol. 174:2234. PubMed
Product Citations
  1. Hilt ZT, et al. 2019. Aging (Albany NY). 11955:11. PubMed
  2. Klemann C, et al. 2009. Am J Pathol. 174:2234. PubMed
RRID
AB_1595502 (BioLegend Cat. No. 644902)

Antigen Details

Structure
Belongs to the nuclear hormone receptor family, NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. RORγ has two isoforms: γ1 and γ2 (also referred to as RORγt). The RORγt differs from the RORγ1 isoform in th
Distribution

Th17 cells

Function
RORγt is essential for lymph nodes and Peyer's patches development. RORγt plays a critical role in control apoptosis during thymopoiesis and T cell homeostasis.
Cell Type
Th17
Biology Area
Cell Biology, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Medvedev A, et al. 1997. Genomics 46:93.
2. He YW, et al. 1998. Immunity 9:797.
3. Eberl G, et al. 2004. Nat. Immunol. 5:64.

Gene ID
19885 View all products for this Gene ID
UniProt
View information about RORgamma on UniProt.org
Go To Top Version: 4    Revision Date: 08.29.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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