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Purified anti-RORγ Antibody

Pricing & Availability
Clone
RORg2 (See other available formats)
Regulatory Status
RUO
Other Names
RORc, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
RORg2_PURE_RORy_Antibody_WB_082018
Total cell lysates (15 µg protein) from mouse thymus (lane 1, 2), mouse spleen (lane 3) and Molt-4 cells (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1) or 2 µg/mL (1:250 dilution, lane 2, 3, 4) of purified anti-RORγ antibody, clone RORg2 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-hamster-IgG secondary antibody conjugated to HRP for purified anti-RORγ antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • RORg2_PURE_RORy_Antibody_WB_082018
    Total cell lysates (15 µg protein) from mouse thymus (lane 1, 2), mouse spleen (lane 3) and Molt-4 cells (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1) or 2 µg/mL (1:250 dilution, lane 2, 3, 4) of purified anti-RORγ antibody, clone RORg2 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-hamster-IgG secondary antibody conjugated to HRP for purified anti-RORγ antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
646501 25 µg 147 CHF
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646502 100 µg 323 CHF
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Description

RORγ (Retinoid-related orphan receptor gamma) belongs to nuclear hormone receptor family, NR1 subfamily. RORγ has two isoforms: RORγ1 and RORγ2 (also known as RORγt). RORγ1 contains 516 amino acids and RORγt contains 495 amino acids (RORγt lacks the amino terminus). RORγ1 is expressed in thymus, kidney, liver, muscle and brown fat tissue but not white fat tissue. RORγt is specifically expressed in CD4+/CD8+ thymocytes and lymphoid tissue inducers (LTi). RORγ plays critical roles during thymopoiesis, T cell homeostasis, lymph nodes and Peyers patch development. Among two isoforms, RORγt is well studied and it promotes thymocyte differentiation into pro-inflammatory Th17 cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse RORγ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sun Z, et al. 2000. Science 288:2369
Product Citations
  1. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  2. Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed
  3. Morita Y, et al. 2022. Bone Res. 10:56. PubMed
RRID
AB_2180302 (BioLegend Cat. No. 646501)
AB_2180302 (BioLegend Cat. No. 646502)

Antigen Details

Structure
RORγ has two isoforms: RORγ1 and RORγ2 (also known as RORγt). RORγ1 contains 516 amino acids and RORγt contains 495 amino acids (RORγt lacks the amino terminus).
Distribution

RORγ1 is expressed in thymus, kidney, liver, muscle and brown fat tissue but not white fat tissue. RORγt is specifically expressed in CD4+/CD8+ double positive thymocytes, Th17 cells and lymphoid tissue inducers (LTi).

Function
RORγ plays critical roles during thymopoiesis, T cell homeostasis, lymph nodes and Peyers patch development. Among two isoforms, RORγt is well studied and it promotes thymocyte differentiation into pro-inflammatory Th17 cells.
Cell Type
Thymocytes
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Medvedev A, et al. 1997. Genomics 46:93.
2. He YW, et al. 1998. Immunity 9:797.
3. Eberl G, et al. 2004. Nat. Immunol. 5:64.

Gene ID
19885 View all products for this Gene ID
UniProt
View information about RORgamma on UniProt.org
Go To Top Version: 2    Revision Date: 08.20.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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