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Purified anti-RPA32/RPA2 Antibody

Pricing & Availability
Clone
W18238A (See other available formats)
Regulatory Status
RUO
Other Names
Replication Protein A2, Replication Protein A 32 kDa Subunit, Replication Factor A Protein 2, RF-A Protein 2, RP-A P32, REPA2, Replication Protein A2 (32kD), RPA34
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18238A_PURE_RPA32-RPA2_Antibody_1_021820
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-RPA32/RPA2 antibody (clone W18238A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18238A_PURE_RPA32-RPA2_Antibody_1_021820
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-RPA32/RPA2 antibody (clone W18238A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18238A_PURE_RPA32-RPA2_Antibody_2_021820
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 1.0 µg/mL (1:500 dilution) of either purified rat IgG2a, κ Isotype ctrl antibody (Cat. No. 400502) (panel A) or purified anti-RPA32/RPA2 antibody (clone W18238A) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • W18238A_PURE_RPA32-RPA2_Antibody_3_022520
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2a, κ Isotype ctrl antibody (Cat. No. 400502) or purified anti-RPA32/RPA2 antibody (clone W18238A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a control antibody against a separate epitope of RPA32/RPA2. Lane M: Molecular weight marker.
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935501 25 µg 101 CHF
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935502 100 µg 253 CHF
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Description

Replication protein A (RPA) is a single-stranded DNA-binding protein comprised of three subunits of 70, 32, and 14 kD, and is involved in the regulation of DNA replication, repair, and homologous recombination. During replication, RPA stabilizes single-stranded DNA by protecting it from nucleases and preventing the formation of secondary structures. Under genotoxic stress conditions, the RPA32 subunit is phosphorylated by the PI3K family of kinases including ATM, ATR, and DNA-PK. Hyperphosphorylated RPA32 has shown to promote homologous recombination by recruiting Rad51, which is required for homologous pairing and strand exchange reactions during DNA repair.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length recombinant human RPA2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse RPA2.

Triton X-100 permeabilization produced stronger staining than methanol permeabilization in ICC testing.

RRID
AB_2832896 (BioLegend Cat. No. 935501)
AB_2832896 (BioLegend Cat. No. 935502)

Antigen Details

Structure
RPA2 is a 270 amino acid protein with a predicted molecular weight of 29 kD.
Distribution

Nucleus/ ubiquitously expressed

Function
DNA replication, DNA repair
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Antigen References
  1. Wold M. 1997. Annu Rev Biochem. 66:61.
  2. Yoo E, et al. 2005. Oncogene. 24:5423.
  3. Shi W, et al. 2010. Carcinogenesis. 31:994.
  4. Lee D, et al. 2010. Nat Struct Mol Biol. 17:365.
  5. Zuazua-Villar P, et al. 2015. Nucleic Acids Res. 43:9776.
Gene ID
6118 View all products for this Gene ID
UniProt
View information about RPA32/RPA2 on UniProt.org
Go To Top Version: 1    Revision Date: 02.18.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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