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Purified anti-RSK1 Antibody

Pricing & Availability
Clone
6B9-D6-F8 (See other available formats)
Regulatory Status
RUO
Other Names
Ribosomal protein S6 kinase alpha-1, 90kD ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1, HU-1, P90-RSK1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6B9-D6-F8_PURE_RSK1_Antibody_011222
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RSK1 (clone 6B9-D6-F8) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 6B9-D6-F8_PURE_RSK1_Antibody_011222
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RSK1 (clone 6B9-D6-F8) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
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602551 25 µg 118 CHF
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602552 100 µg 293 CHF
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Description

Ribosomal protein S6 kinase α1 (RPS6KA1), most commonly known as RSK1, is a serine/threonine kinase that acts downstream of ERK/MAPK signaling. Upon activation, RSK1 translocates to the nucleus where it controls the activity of several important transcription factors, such as CREB1 and c-FOS. Additionally, RSK1 directly controls translation through phosphorylation of the transcriptional protein RPS6 and EIF4B. Through these roles, RSK1 is involved in the control of cellular growth and proliferation, and genetic variants of RSK1 have been implicated in multiple cancer types.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant RSK1 fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

 

Application Notes

This clone does not recognize mouse RSK1 in Western Blot

RRID
AB_2910476 (BioLegend Cat. No. 602551)
AB_2910476 (BioLegend Cat. No. 602552)

Antigen Details

Structure
RSK1 is a 735 amino acid protein with a predicted molecular weight of 83 kD.
Distribution

RSK1 is widely expressed in most tissues and cell types with primarily cytosolic localization.

Function
Kinase, regulates transcription and translation
Interaction
SRF, c-Fos, CREB1, ERK, NFATc4, MITF, ER81
Biology Area
Cell Proliferation and Viability, Protein Synthesis, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Anjum R, et al. 2008. Nat Rev Cell Biol. 9:747-58.
Gene ID
6195 View all products for this Gene ID
UniProt
View information about RSK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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