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Purified anti-RRM1 Antibody

Pricing & Availability
Clone
O99A2 (See other available formats)
Regulatory Status
RUO
Other Names
Ribonucleotide Reductase Catalytic Subunit M1, Ribonucleoside-Diphosphate Reductase Large Subunit, Ribonucleoside-Diphosphate Reductase Subunit M1, Ribonucleotide Reductase M1 Polypeptide, EC 1.17.4.1
Isotype
Mouse IgG1, κ
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Product Citations
publications
O99A2_PURE_RRM1_Antibody_1_051520
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RRM1 antibody (clone O99A2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • O99A2_PURE_RRM1_Antibody_1_051520
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RRM1 antibody (clone O99A2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • O99A2_PURE_RRM1_Antibody_2_051520
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 1.0 µg/mL of either purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 401402) (panel A) or purified anti-RRM1 antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
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937101 25 µg 101 CHF
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937102 100 µg 253 CHF
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Description

RRM1 is the large activating subunit of ribonucleotide reductase, an enzyme that converts ribonucleotides into deoxyribonucleotide triphosphates (dNTPs).

The dNTPs formed as a result of this catalytic conversion are used in DNA synthesis and help maintain stable levels of DNA during cell division and DNA repair. Dysregulation of ribonucleotide reductase can result in dNTP imbalances, causing misincorporation of DNA. RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human RRM1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A minor 37kD band was detected by the clone during western blot product development testing. This band was also detected using a control antibody that was generated with a separate RRM1 immunogen; we believe this band represents a minor RRM1 degradation product.

When tested for ICC on HeLa cells fixed with PFA and permeabilized with Triton X-100, this clone failed to stain RRM1. We therefore do not recommend this fixation-permeabilization step when using this clone for ICC.

RRID
AB_2861122 (BioLegend Cat. No. 937101)
AB_2861122 (BioLegend Cat. No. 937102)

Antigen Details

Structure
RRM1 is a 792 amino acid protein with a predicted molecular weight of 90 kD.
Distribution

Cytosol

Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Antigen References
  1. Bepler G, et al. 2006. J Clin Oncol. 24:4731.
  2. Zheng Z, et al. 2007. N Engl J Med. 356:800.
  3. Ceppi P, et al. 2006. Ann Oncol. 17:1818.
Gene ID
6240 View all products for this Gene ID
UniProt
View information about RRM1 on UniProt.org
Go To Top Version: 2    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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