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Purified anti-RUNX2 Antibody

Pricing & Availability
Clone
W15212A (See other available formats)
Regulatory Status
RUO
Other Names
Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1 (CBF-alpha-1), Oncogene AML-3, Osteoblast-specific transcription factor 2 (OSF-2), Polyomavirus enhancer-binding protein 2 alpha A subunit
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W15212A_Purified_RUNX2_Antibody_2_123118.png
Total cell lysates (15 µg total protein) from Saos-2, U937 (reduced expression control), THP-1 (negative control), and THP-1(reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.005 µg/mL (1:100,000 dilution) of Purified anti-RUNX2 Antibody, clone W15212A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 1_W15212A_Purified_RUNX2_Antibody_2_123118.png
    Total cell lysates (15 µg total protein) from Saos-2, U937 (reduced expression control), THP-1 (negative control), and THP-1(reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.005 µg/mL (1:100,000 dilution) of Purified anti-RUNX2 Antibody, clone W15212A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2_W15212A_Purified_RUNX2_Antibody_101816
    Total cell lysate (15 µg/lane) from HOS cells, NIH3T3 cells, and HeLa cells were resolved by 4-12% Bis-tris electrophoresis, transferred to nitrocellulose, and probed with 0.005 µg/mL purified anti-RUNX2 (clone W15212A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
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692802 100 µg 253 CHF
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Description

Runt Related Transcription Factor 2 (RUNX2), also known as CBFA1, belongs to the mammalian Runt-related transcription factor (RUNX) family. The RUNX family is comprised of three members: RUNX1, RUNX2, and RUNX3. The RUNX proteins are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. Among these members, RUNX2 contains one Runt domain and specifically expresses in osteoblasts. It plays critical roles in skeletal mineralization, osteoblast differentiation of mesenchymal stem cells, chondrocyte hypertrophy, endothelial cell migration, and vascular invasion of developing bones. Defects in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) and defective endochondral and intramembranous bone formation. In some cases, defects in RUNX2 are exclusively associated with dental anomalies. RUNX2 binds DNA as a monomer or as a subunit of a heterodimeric complex. In osteoblasts, RUNX2 supports transcriptional activation by synergizing with SPEN/MINT. RUNX2 also functions to inhibit KAT6B (MYST4)-dependent transcriptional activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human RUNX2 recombinant protein (237-422 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.002 - 0.01 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes multiple isoforms of RUNX2.

Product Citations
  1. Ebata T, et al. 2021. iScience. 24(6):102643. PubMed
RRID
AB_2632769 (BioLegend Cat. No. 692802)

Antigen Details

Structure
521 amino acids with a predicted molecular weight of 56.6 kD. Contains a Runt domain responsible for DNA-binding and protein-protein interaction.
Distribution

Nucleus.

Function
RUNX2 is a transcription factor and has been found to be involved in the regulation of skeletal gene expression, osteoblastic differentiation, and bone morphogenesis.
Interaction
Interacts with Ku70, Ku80, HIVEP3, IFI204, SATB2, HIPK3, DDX5, FOXO1, CCNB1, KAT6A, KAT6B, FOXP3, and TMEM119.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Kidwai F, et al. 2016. Stem Cells. 34:2079.
2. Tandon M, et al. 2016. Oncogene. 35:4730.
3. Owens TW, et al. 2014. Cancer. Res. 74:5277.
4. Sawai CM, et al. 2013. J. Exp. Med. 210:2151.
5. Komori T. 2011. J. Cell. Biochem. 112:750.
6. Westendorff JJ. 2006. J. Cell. Biochem. 98:54-64.

Gene ID
860 View all products for this Gene ID
UniProt
View information about RUNX2 on UniProt.org
Go To Top Version: 2    Revision Date: 12.31.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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