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Purified anti-SARS-CoV-2 S Protein S1 Antibody

Pricing & Availability
Clone
A20103O (See other available formats)
Regulatory Status
RUO
Other Names
S1, Spike Protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20103O_PURE_SARS_CoV-2-S_Protein-S1_Antibody_1_033121
His-tagged SARS-CoV-2 S protein S1 transfected CHO cells were stained with anti-S1 (clone A20103O) purified (left) or rat IgG2a, κ isotype control (clone RTK2758) purified (right) followed by anti-rat IgG PE (clone Poly4054) and Alexa Fluor® 647 anti-His Tag (clone J095G46).
  • A20103O_PURE_SARS_CoV-2-S_Protein-S1_Antibody_1_033121
    His-tagged SARS-CoV-2 S protein S1 transfected CHO cells were stained with anti-S1 (clone A20103O) purified (left) or rat IgG2a, κ isotype control (clone RTK2758) purified (right) followed by anti-rat IgG PE (clone Poly4054) and Alexa Fluor® 647 anti-His Tag (clone J095G46).
  • A20103O_PURE_SARS_CoV-2-S_Protein-S1_Antibody_2_031721
    Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of His-tagged recombinant SARS-CoV-2 S Protein S1 (Cat. No. 792906) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SARS-CoV-2 S Protein S1 antibody (clone A20103O) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-human IgG antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • A20103O_PURE_SARS_CoV-2-S_Protein-S1_Antibody_3_031721
    200 ng of recombinant SARS-CoV-2 S protein S1 + S2 (Cat. No. 793704) was coated onto a Costar™ 96-well high binding assay plate and incubated with a dilution series of purified anti-SARS-CoV-2 S protein S1 antibody (clone A20103O). Bound antibodies were detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm.
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945101 25 µg 112 CHF
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945102 100 µg 335 CHF
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Description

SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). Three S1/S2 heterodimers assemble to form a trimer spike protruding from the viral envelope. The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. Triggered by receptor binding, proteolytic processing and/or acidic pH in the cellular compartments, the class I viral fusion protein undergoes a transition from a metastable pre-fusion state to a stable post-fusion state during infection, in which the receptor-binding subunit is cleaved, and the fusion subunit undergoes large-scale conformational rearrangements to expose the hydrophobic fusion peptide, induce the formation of a six-helix bundle, and bring the viral and cellular membranes close for fusion. The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant SARS-CoV-2 S protein corresponding to S1 subunit
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA, FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For Direct ELISA, a concentration of 114.9 ng/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.

Product Citations
  1. Mamedov T, et al. 2021. Viruses. 13:. PubMed
RRID
AB_2890875 (BioLegend Cat. No. 945101)
AB_2890875 (BioLegend Cat. No. 945102)

Antigen Details

Structure
Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits.
Distribution

Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane 

Function
Mediates fusion between virus and host cell membranes
Interaction
ACE2
Ligand/Receptor
ACE2
Biology Area
COVID-19
Antigen References

1. Walls AC, et al. 2020. Cell. 181(2):281-292.
2. Yan R, et al. 2020. Science. 367 (6485):1444-1448.
3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263.
4. Shang J, et al. 2020. PNAS. 117(21):11727-11734

Gene ID
43740568 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org
Go To Top Version: 2    Revision Date: 03.31.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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