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- Clone
- O96C6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Special AT-rich sequence-binding protein 1, SATB homeobox 1, DNA-binding protein SATB1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total cell lysate from Jurkat (lane 1, 15 µg), mouse thymus (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-SATB1 (clone O96C6) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection. -
Jurkat cells were stained with purified anti-SATB1 (clone O96C6) antibody, followed by staining with Alexa Fluor® 594 conjugated goat anti-mouse IgG antibody (red, Cat. No. 405326). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 676102 | 100 µg | 288 CHF | ||||
SATB1 is a global regulator that selectively tethers multiple genomic loci and recruits chromatin-remodeling enzymes (e.g. ACF, ISWI, HDAC1 and histone acetyltransferase) to control chromatin structure and gene expression. SATB1 also recruits corepressors or coactivators to promoters. These two opposing interactions, however, are determined by whether or not the SATB1 is phosphorylated by protein kinase C. Unphosphorylated and phosphorylated SATB1 preferably binds to PCAF and HDAC1, respectively. SATB1 also forms a regulatory complex with the promyelocytic leukaemia (PML) protein to organize the major histocompatibility complex (MHC) class I locus into distinct chromatin-loop architecture and mediate the expression of a subset of MHC class I genes. Upon Th2 cell activation, SATB1 expression is induced to compact chromatin into dense-loop structure and to prompt the expression of IL-4, IL-5, IL-13 and c-Maf. SATB1 is also reported as being crucial for the growth and metastasis of breast cancer and is a potential prognostic marker.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length recombinant human SATB1 (NP_002962) produced in HEK293T cells.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Affinity purified.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2565610 (BioLegend Cat. No. 676102)
Antigen Details
- Structure
- Homodimer. Canonical sequence contains 763 amino acids, predicted molecular weight of 86 kD. Isoform contains 795 amino acids, predicted molecular weight of 89 kD.
- Distribution
-
Nucleus matrix. Nucleus. Promyelocytic leukemia nuclear bodies (SUMOylated).
- Function
- SATB1 recruits chromatin remodeling factors to regulate chromatin-loop structure and gene transcription. It is an important silencing factor for initiating the Xist RNA-mediated X inactivation in mammals.
- Interaction
- CUX1, CtBP1, PCAF, SUMOylated PML, HDAC1 and HIV-1 Tat, DYNLT2, POLR2J2, EP300, special AT-rich DNA sequences (consensus SATB1-binding sequence, CSBS).
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Antigen References
-
1. Agrelo R, et al. 2009. Dev. Cell. 16:507.
2. Han HJ, et al. 2008. Nature. 452:187.
3. Cai S, et al. 2006. Nature Genet. 38:1278.
4. Pavan Kumar, P, et al. 2006. Mol. Cell. 22:231.
5. Fujii Y, et al. 2003. Microbiol. Immunol. 47:803.
6. Yasui, D, et al. 2002. Nature. 419:641. - Gene ID
- 6304 View all products for this Gene ID
- UniProt
- View information about SATB1 on UniProt.org
Related FAQs
Other Formats
View All SATB1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SATB1 | O96C6 | WB,ICC |
| Alexa Fluor® 594 anti-SATB1 | O96C6 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-SATB1
Jurkat cells were stained with purified anti-SATB1 (clone O9... 
Total cell lysate from Jurkat (lane 1, 15 µg), mouse thymus ... -
Alexa Fluor® 594 anti-SATB1
Jurkat cells were fixed with 1% paraformaldehyde (PFA) for t...
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