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Purified anti-Serpin E1 Antibody

Pricing & Availability
Clone
A16081E (See other available formats)
Regulatory Status
RUO
Other Names
Plasminogen activator inhibitor type 1 (PAI-1, PLANH1), Serine (Or Cysteine) Proteinase Inhibitor, Endothelial Plasminogen Activator Inhibitor
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16081E_PURE_Serpin-E1_Antibody_020623
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-Serpin E1 (clone A16081E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A16081E_PURE_Serpin-E1_Antibody_020623
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-Serpin E1 (clone A16081E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A16081E_PURE_Serpin-E1_Antibody_2_020623
    IHC staining of purified anti-Serpin E1 (clone A16081E) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated without (panel A) or with (panel B) 10 µg/mL of antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801) and the image was captured with a 40X objective. Scale bar: 50 µm
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610751 25 µg 120 CHF
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610752 100 µg 370 CHF
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Description

Serpin E1 is a member of the serine proteinase inhibitors (serpin) superfamily, which is divided into two groups: inhibitory and non- inhibitory serpins. Serpin E1 is the most important physiological inhibitor of tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA), and therefore is a key regulator of fibrinolysis via inhibition of the activation of the broad-spectrum serine protease plasmin. Serpin E1 forms a covalent bond with both t-PA and u-PA (1:1 molar ratio). It is known that several receptors (uPAR, LRP-1A, megalin, VLDLR, and LRP-1B) bind and mediate endocytosis of plasminogen activator-Serpin E1 complexes. Serpin E1 is present in blood and the extracellular matrix in a complex with vitronectin. Elevated Serpin E1 levels have been implicated in the pathogenesis of atherosclerosis and myocardial infarction (MI). High levels of plasma Serpin E1 in patients precede MI. Abnormal levels of Serpin E1 is also associated with metabolic syndrome, obesity, obstructive nephropathy, cancer, cardiac fibrosis, pulmonary fibrosis, and bleeding thrombosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Serpin E1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

Clone A16081E is not suitable for ICC.

RRID
AB_2936732 (BioLegend Cat. No. 610751)
AB_2936732 (BioLegend Cat. No. 610752)

Antigen Details

Structure
Serpin E1 is a 402 amino-acid protein with a predicted molecular weight of 45 kD.
Distribution

Cytoplasmic, secreted

Function
Serpins bind to and inhibit proteinases
Interaction
Serpin E1 binds to and inhibits urokinase and tissue plasminogen activator.
Biology Area
Cardiovascular Biology, Cell Adhesion, Inhibitory Molecules
Antigen References
  1. van Mourik JA, et al. 1984. J Biol Chem. 259:14914-21.
  2. Zhou A, et al. 2003. Nat Struct Biol. 10:541-4.
  3. Tsantes AE, et al. 2008. Thromb Res. 122:736-42.
  4. Dupon DM, et al. 2009. Front Biosciences 14:1337-61.
  5. Ploplis VA. 2011. Curr Drug Targets. 12:1782-9.
  6. Yasar Yildiz S, et al. 2014. Scientific World Journal. 2014:858293.
Gene ID
5054 View all products for this Gene ID
UniProt
View information about Serpin E1 on UniProt.org
Go To Top Version: 1    Revision Date: 02.06.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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