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Purified anti-SH2D1A (SAP) Antibody

Pricing & Availability
Clone
1A9 (See other available formats)
Regulatory Status
RUO
Other Names
SH2 Domain Containing 1A, SAP, T-Cell Signal Transduction Molecule SAP, SLAM-Associated Protein, T Cell Signal Transduction Molecule SAP, MTCP1, XLPD
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1A9_PURE_SH2D1A_Antibody_WB_091916
Total cell lysates (15 µg protein) from Jurkat (lane 1), mouse thymus (lane 2) and HeLa (lane 3, negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-SH2D1A (clone 1A9) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
  • 1A9_PURE_SH2D1A_Antibody_WB_091916
    Total cell lysates (15 µg protein) from Jurkat (lane 1), mouse thymus (lane 2) and HeLa (lane 3, negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-SH2D1A (clone 1A9) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
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690702 100 µg 253 CHF
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Description

SH2D1A, also known as SAP, is a small adaptor protein which plays a major role in the bidirectional stimulation of T and B cells. SH2D1A is predominantly expressed in T cells and NK cells, and functions to regulate signal transduction pathways downstream of the SLAM family of surface receptors. The SH2D1A gene is frequently mutated in patients with X-linked lymphoproliferative disease. Malfunction of the SLAM-SAP pathway results in autoimmune diseases, tumors of the lymphoid tissues and inadequate responses to infectious agents.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length purified human SH2D1A.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629824 (BioLegend Cat. No. 690702)

Antigen Details

Structure
128 amino acids with a characteristic of SH2 domain. The predicted molecular weight is approximately 14kD.
Distribution
T cells, NK cells and monocytes.
Function
Stimulation of T and B cells.
Interaction
SLAM family, SH2D1B, and FYN.
Ligand/Receptor
SLAM family; SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7.
Cell Type
T cells, NK cells, Monocytes
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Tangye SG. 2014. J. Clin. Immunol. 34:772.
2. Yang X, et al. 2012. Pediatr. Int. 54:447.
3. Ma CS, Deenick EK. 2011. Ann. N Y Acad. Sci. 1217:32-44.
4. Detre C, et al. 2010. Semin. Immunopathol. 32:157.
5. Vilar ML, et al. 2011. Braz. J. Med. Biol. Res. 44:276.

Gene ID
4068 View all products for this Gene ID
UniProt
View information about SH2D1A on UniProt.org
Go To Top Version: 1    Revision Date: 09.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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