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Purified anti-SIK2 Antibody

Pricing & Availability
Clone
S15G10 (See other available formats)
Regulatory Status
RUO
Other Names
Serine/threonine protein kinase SNF1 like kinase 2, Qin-induced kinase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S15G10_PURE_SIK2_Antibody_1_112818_updated
Total cell lysates (15 µg total protein) from HEK293 and HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SIK2 Antibody, clone S15G10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker.
  • S15G10_PURE_SIK2_Antibody_1_112818_updated
    Total cell lysates (15 µg total protein) from HEK293 and HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SIK2 Antibody, clone S15G10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker.
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636702 100 µg 229 CHF
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Description

SIK2 (Salt inducible kinase 2) belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and AMP-activated protein kinase (AMPK) subfamily, predicted molecular weight 104 kD. SIK2 is found in adipocytes and might be involved in metabolic regulation in adipose tissue. Sik2 phosphorylates ser794 of human insulin receptor substrate-1 (IRS1) in insulin-stimulated adipocytes, playing important roles in modulating the insulin-signaling cascade of adipocytes. Clone S15G10 has been shown to be useful for western blotting, immunoprecipitation, and immunoflorence staining of human SIK2 protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human SIK2
Molecular Mass
104
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested working dilution is 1:250. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Bon H, et al. 2015. Mol Cancer Res. 13:620. PubMed
Product Citations
  1. Bon H, et al. 2015. Mol Cancer Res. 13:620. PubMed
  2. Maxfield K, et al. 2016. Mol Cell Biol. 36(24):3048-3057. PubMed
RRID
AB_2187452 (BioLegend Cat. No. 636702)

Antigen Details

Structure
Belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and AMP-activated protein kinase (AMPK) subfamily. SIK2 contains 1 protein kinase domain and 1 UBA domain.
Interaction
IRS 1, TORC2
Bioactivity
Involved in metabolic regulation in adipose tissue. Sik2 phosphorylates ser794 of human insulin receptor substrate-1(IRS1) in insulin-stimulated adipocytes, playing important roles in modulating the insulin-signaling cascade of adipocytes.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Horike N, et al. 2003. J. Biol. Chem. 278:18440.

Gene ID
23235 View all products for this Gene ID
UniProt
View information about SIK2 on UniProt.org
Go To Top Version: 5    Revision Date: 11.28.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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