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Purified anti-SLP76 Phospho (Ser376) Antibody

Pricing & Availability
Clone
A18001A (See other available formats)
Regulatory Status
RUO
Other Names
LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18001A_Purified_SLP76_Phospho_Antibody_020719.png
Whole cell extracts (15 µg protein) from serum starved Jurkat and EL4 cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-SLP76 Phospho (Ser376), clone A18001A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal SLP76 loading was confirmed by probing membranes with anti-pan SLP76 Antibody, clone H76 (Cat. No. 625002) at a 1:1000 dilution. Lane M: Molecular Weight marker.
  • A18001A_Purified_SLP76_Phospho_Antibody_020719.png
    Whole cell extracts (15 µg protein) from serum starved Jurkat and EL4 cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-SLP76 Phospho (Ser376), clone A18001A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal SLP76 loading was confirmed by probing membranes with anti-pan SLP76 Antibody, clone H76 (Cat. No. 625002) at a 1:1000 dilution. Lane M: Molecular Weight marker.
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667101 25 µg 112 CHF
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667102 100 µg 265 CHF
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Description

SLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being tranlocated to the plasma membrane and facilitating the assembly of a multi-subunit complex comprised of Vav1, Nck, ITK, and PLCγ1. SLP76 also plays an essential role in normal T cell development.

SLP76 also represents a negative regulatory node in the TCR activation pathway. Following TCR engagement, SLP76 is phosphorylated at Ser376 by the hematopoietic progenitor kinase (HPK1). This modification promotes an interaction between SLP76 and the scaffolding protein 14-3-3ε, resulting in dissociation of the TCR signaling complex and a diminution of TCR signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic human SLP76 peptide containing phosphorylated Ser376
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on PFA-fixed and methanol permeabilized Jurkat cells treated with or without H2O2 and produced nuclear staining that was not responsive to the treatment. We therefore do not recommend this clone for ICC.

†cross-reactivity to Mouse confirmed based on Western blot testing.

RRID
AB_2801128 (BioLegend Cat. No. 667101)
AB_2801128 (BioLegend Cat. No. 667102)

Antigen Details

Structure
SLP76 is a 533 amino acid protein with a predicted molecular weight of ~60 kDa.
Distribution

Hematopoietic cells/cytosol

Function
Adapter protein/TCR Signaling
Interaction
ITK, LAT, GRB2, PLCγ1, NCK, VAV, ITK, LCK, 14-3-3ε
Cell Type
B cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Sauer K, et al. 2001. J Biol Chem. 276:45207.
  2. Koretzky GA, et al.. 2006. Nat Rev Immunol. 6:67.
  3. Di Bartolo V, et al. 2007. J Exp Med. 204:681.
  4. Lasserre, et al. 2011. J Cell Biol. 195:839.

 

Gene ID
3937 View all products for this Gene ID
UniProt
View information about SLP76 Phospho Ser376 on UniProt.org
Go To Top Version: 1    Revision Date: 02.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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