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Purified anti-SMYD2 Antibody

Pricing & Availability
Clone
1G10-E11-H9 (See other available formats)
Regulatory Status
RUO
Other Names
SET and MYND domain-containing protein 2, Histone methyltransferase SMYD2, HSKM-B, Lysine N-methyltransferase 3C, N-lysine methyltransferase SMYD2, ZMYND14, KMT3C
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_1G10_E11_H9_PURE_SMYD2_Antibody_IHC_112222
IHC staining of purified anti-SMYD2 (clone 1G10-E11-H9) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Tris EDTA, pH 9.0, the tissue was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_1G10_E11_H9_PURE_SMYD2_Antibody_IHC_112222
    IHC staining of purified anti-SMYD2 (clone 1G10-E11-H9) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Tris EDTA, pH 9.0, the tissue was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_1G10_E11_H9_PURE_SMYD2_Antibody_WB_112222
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-SMYD2 (clone 1G10-E11-H9) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
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609451 25 µg 118 CHF
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609452 100 µg 293 CHF
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Description

SET and MYND domain-containing protein 2, also known as SMYD2, is one of five members of the SMYD family of lysine methyltransferases composed of an evolutionarily conserved SET domain and a zinc finger-containing MYND domain. SMYD2 localizes to the nucleus and cytoplasm and methylates histones such as H2B, H3K4, H3K36, and H4 as well as non-histone substrates, notably p53 on lysine 370. As a protein lysine methyltransferase, SMYD2 is a regulator of transcription and epigenetic signaling and functions in the regulation of cell cycle and apoptosis. It has also been shown to promote NF-κB signaling pathways via methylation of TRAF2 and STAT3. Aberrant SMYD2 expression is associated with a variety of cancers and other diseases including kidney disease, cardiovascular disease, and rheumatoid arthritis. It promotes tumorigenesis and metastasis through the repression of tumor suppressor proteins RB1, p53, and PTEN.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human SMYD2 protein fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 1G10-E11-H9 is not recommended for testing in mouse.

RRID
AB_2928003 (BioLegend Cat. No. 609451)
AB_2928003 (BioLegend Cat. No. 609452)

Antigen Details

Structure
SMYD2 is a 433 amino acid protein with a predicted molecular weight of 50 kD
Distribution

Ubiquitously expressed/Cytoplasm, Nucleus

Function
Chromatin regulator, Methyltransferase,Transcription
Interaction
P53, RB1, H2B, H4, H3K36, H2K4, HSP90/p23, PTEN, ERα, ALK, PARP1, MAPKAPK3, STAT3
Ligand/Receptor
Metal-binding, S-adenosyl-L-methionine, Zinc
Biology Area
Cell Biology
Molecular Family
Adaptor Proteins, Adhesion Molecules, Scaffold Proteins
Antigen References
  1. Abu-Farha M, et al. 2008. Mol Cell Proteomics. 7:560-72.
  2. Ferguson AD, et al. 2011. Structure. 19:1262-73.
  3. Wu W, et al. 2021. Clin Transl Med. 11:e591.
  4. Yi X, et al. 2019. Clin Epigenetics. 11:112.
Gene ID
56950 View all products for this Gene ID
UniProt
View information about SMYD2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.22.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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