- Clone
- 14A6A34 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_14A6A34_Purified_SOX2_Antibody_WB_1_120417 1_14A6A34_Purified_SOX2_Antibody_WB_1_120417](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/14A6A34_Purified_SOX2_Antibody_WB_1_120417.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total lysates (15 µg protein) from HeLa (Low expression negative control) and NTERA-2 (Positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 (0.25 µg/ml), and 1:1000 (0.5 µg/ml) purified anti-SOX2 antibody, clone 14A6A34. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution). -
Total cell lysate from NTERA-2 cells (lane 1, 15 µg), NF-1 cells (lane 2, 15 µg) and HeLa cells (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-SOX2 antibody (clone 14A6A34). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. -
NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL SOX2 Antibody (14A6A34) and followed by DyLight™ 594 goat anti-mouse IgG (red) for 1 hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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656102 | 100 µg | 311 CHF |
SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human SOX recombinant protein
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Affinity purified
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 0.5 µg per ml (1:1000-1:2000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Product Citations
-
- RRID
-
AB_2562246 (BioLegend Cat. No. 656102)
Antigen Details
- Structure
- 317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
- Distribution
-
Nucleus
- Function
- Transcription factor that regulates the expression of the genes involved in embryonic development
- Interaction
- Interacts with FGFR1, SOX3, and ZSCAN10
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
- Antigen References
-
1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228.
2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725.
3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772.
4. Jeong CH, et al. 2010. Stem Cells 28:2141.
5. Xiang R, et al. 2011. Br. J. Cancer 104:1410.
6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426. - Gene ID
- 6657 View all products for this Gene ID
- UniProt
- View information about SOX2 on UniProt.org
Other Formats
View All SOX2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SOX2 | 14A6A34 | WB,ICC |
PE anti-SOX2 | 14A6A34 | ICFC |
Alexa Fluor® 594 anti-SOX2 | 14A6A34 | ICC |
Alexa Fluor® 488 anti-SOX2 | 14A6A34 | ICFC,ICC |
Alexa Fluor® 647 anti-SOX2 | 14A6A34 | ICC,FC |
Pacific Blue™ anti-SOX2 | 14A6A34 | ICFC |
Brilliant Violet 421™ anti-SOX2 | 14A6A34 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-SOX2
Total cell lysate from NTERA-2 cells (lane 1, 15 µg), NF-1 c... NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for ... Total lysates (15 µg protein) from HeLa (Low expression nega... -
PE anti-SOX2
Human embryonic carcinoma NCCIT cells were fixed an... -
Alexa Fluor® 594 anti-SOX2
NCCIT cells were fixed with 1% paraformaldehyde (PFA) for 10... -
Alexa Fluor® 488 anti-SOX2
Human embryonic carcinoma NCCIT cells were fixed an... NCCIT cells were fixed with 1% paraformaldehyde (PF... -
Alexa Fluor® 647 anti-SOX2
Human embryonic carcinoma NCCIT cells were fixed and permeab... NCCIT cells were fixed with 1% paraformaldehyde (PFA) for 10... -
Pacific Blue™ anti-SOX2
Human embryonic carcinoma NCCIT cells were fixed an... -
Brilliant Violet 421™ anti-SOX2
NCCIT cells were fixed with 1% paraformaldehyde (PFA) for te...
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