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Purified anti-SPI1 (PU.1) Antibody

Pricing & Availability
Clone
7C6B05 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen focus forming virus (SFFV) proviral integration oncogene, Transcription factor PU.1
Isotype
Mouse IgG1, κ
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Product Citations
publications
7C6B05_PURE_SPI1-PU1_Antibody_1_WB_070918
Total cell lysates (15 µg protein) from THP1 (lane 1) and MCF-7 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-SPI1 (PU.1) Antibody, clone 7C6B05 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-SPI1 (PU.1) Antibody or 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (lower panel) as a loading control. Lane M: Molecular weight ladder.
  • 7C6B05_PURE_SPI1-PU1_Antibody_1_WB_070918
    Total cell lysates (15 µg protein) from THP1 (lane 1) and MCF-7 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-SPI1 (PU.1) Antibody, clone 7C6B05 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-SPI1 (PU.1) Antibody or 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (lower panel) as a loading control. Lane M: Molecular weight ladder.
  • 7C6B05_PURE_SPI1-PU1_Antibody_2_ICC_070918
    THP-1 cells were stained with purified anti-PU.1 (clone 7C6B05) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • 7C6B05_PURE_SPI1-PU1_Antibody_3_IP_070918
    Immunoprecipitation of PU.1 from THP-1 cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-PU.1 antibody (clone 7C6B05, lane 4). Western blot was performed using anti-PU.1 antibody (clone 7C6B05). The astrostar indicates immunoglobulin heavy chain and light chain.
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658002 100 µg 270 CHF
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Description

SPI1 is a transcription factor belonging to the E26-transformation-specific (Ets) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as occurring malignancy. Overexpression of SPI1 blocks erythroid differentiation and inhibits cell death.  Mice carrying a mutant SPI1 allele show decreased SPI1 expression and develop acute myeloid leukaemia (AML), suggesting the role of SPI1 in oncogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SPI1 recombinant protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations
  1. Breitmeyer R, et al. 2023. Commun Biol. 6:472. PubMed
  2. Yang L, et al. 2020. Cell Stem Cell. 27(1):125-136. PubMed
  3. Guttikonda SR, et al. 2021. Nat Neurosci. 24:343. PubMed
  4. Panagiotakopoulou V, et al. 2020. Nat Commun. 4.04375. PubMed
RRID
AB_2562720 (BioLegend Cat. No. 658002)

Antigen Details

Structure
270 amino acids, predicted molecular weight of 31 kD; contains a C-termianl ETS domain responsible for DNA binding.
Distribution

Nucleus.

Function
SPI1 is a transcription factor required for the development of lymphoid and myeloid cells.
Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmelé P, et al. 2010. Cancer Res. 70:6757.

Gene ID
6688 View all products for this Gene ID
UniProt
View information about SPI1 on UniProt.org
Go To Top Version: 5    Revision Date: 07.09.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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