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Purified anti-STIM1 Antibody

Pricing & Availability
Clone
W18094A (See other available formats)
Regulatory Status
RUO
Other Names
Stromal Interaction Molecule 1, GOK, IMD10, TAM1, STRMK
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W18094A_PURE_STIM1_Antibody_1_070519
Whole cell extracts (15 µg protein) from NTERA-2 (reduced expression control), HeLa, HEK-293, A549 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-STIM1 Antibody, clone W18094A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W18094A_PURE_STIM1_Antibody_1_070519
    Whole cell extracts (15 µg protein) from NTERA-2 (reduced expression control), HeLa, HEK-293, A549 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-STIM1 Antibody, clone W18094A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W18094A_PURE_STIM1_Antibody_2_070519
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602, panel A) or Purified anti-STIM1 Antibody, clone W18094A (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
  • W18094A_PURE_STIM1_Antibody_3_070519
    Whole cell extracts (300 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602) or Purified anti-STIM1 Antibody, clone W18094A. The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with an anti-STIM1 antibody that recognizes a different epitope than W18094A. Lane M: Molecular Weight marker.
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621701 25 µg 101 CHF
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621702 100 µg 253 CHF
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Description

Stromal Interaction Molecule 1, or STIM1 is a single pass transmembrane, resident ER protein that facilitates extracellular calcium uptake following the depletion of intracellular stores. STIM1 directly senses loss of ER-lumenal calcium via an EF hand domain; elevated ER calcium levels stimulate redistribution of STIM1 to close proximity to the plasma mebrane, where it activates ORAI1, a pore component of the store-operated calcium entry system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human STIM1 protein corresponding to amino acid residues 436-685
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When testing W18094A testing for ICC, we observed poor staining of PFA-fixed cells that were permeabilized with methanol.

When testing W18094A for WB, a minor non-specific band of ~50 kD was observed by WB at higher antibody concentrations.

RRID
AB_2814470 (BioLegend Cat. No. 621701)
AB_2814470 (BioLegend Cat. No. 621702)

Antigen Details

Structure
STIM1 is a 685 amino acid protein with a predicted molecular weight of 77 kD.
Distribution

ER Membrane/Ubiquitously expressed

Function
Calcium sensing and uptake
Biology Area
Cell Biology
Molecular Family
Innate Immune Signaling, Ion Channels
Antigen References
  1. Roos J, et al. 2005. J. Cell Biol. 169:435.
  2. Peinelt C, et al. 2006. Nat Cell Biol. 8:771.
  3. Baba Y, et al. 2006. Proc Natl Acad Sci USA. 103:16704.
Gene ID
6786 View all products for this Gene ID
UniProt
View information about STIM1 on UniProt.org
Go To Top Version: 1    Revision Date: 07.05.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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