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Purified anti-Synaptobrevin (VAMP) Antibody

Pricing & Availability
Clone
SP10 (See other available formats)
Regulatory Status
RUO
Other Names
Vesicle-associated membrane protein 1, synaptobrevin-1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
SP10_Purified_Synaptobrevin_Antibody_1_062119
Western blot of purified anti-Synaptobrevin (VAMP) antibody (clone SP10). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • SP10_Purified_Synaptobrevin_Antibody_1_062119
    Western blot of purified anti-Synaptobrevin (VAMP) antibody (clone SP10). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • SP10_Purified_Synaptobrevin_Antibody_2_062119
    IHC staining of purified anti-Synaptobrevin (VAMP) antibody (clone SP10) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP10_Purified_Synaptobrevin_Antibody_3_062119
    IHC staining of purified anti-Synaptobrevin (VAMP) antibody (clone SP10) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • SP10_Purified_Synaptobrevin_Antibody_4_062119
    IHC staining of purified anti-Synaptobrevin (VAMP) antibody (clone SP10) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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837003 25 µg 106 CHF
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837004 100 µg 276 CHF
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Description

Synaptobrevins, also known as VAMPs, along with syntaxins and the synaptosomal-associated protein SNAP25, form the protein complex that is involved with the docking and fusion of synaptic vesicles with the presynaptic membrane. Synaptobrevins have a molecular weight of 18 kD, and are also involved in the formation of the SNARE complexes. The synaptobrevins are degraded by the protein tetanospasmin, from the tetanus-causing bacterium Clostridium tetani, as well as botulinum toxin from Clostridium botulinum.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a crude synaptic preparation of post mortem human brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
ELISA, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Sawada K, et al. 2002. Mol Psychiatry 7:484-92. (WB, IHC-P, ELISA)
  2. Honer WG, et al. 2002. Cerebral Cortex 12:349-56. (WB, ELISA, ICC)
  3. Honer WG, et al. 1993. Brain Res. 609:9-20. (ICC)
  4. Poon VY, et al. 2014. Front. Cell Neurosci. 8:66. (ICC) PubMed
RRID
AB_2810717 (BioLegend Cat. No. 837003)
AB_2810717 (BioLegend Cat. No. 837004)

Antigen Details

Structure
Synaptobrevin 1 (VAMP1) is a 118 amino acid protein with an apparent molecular mass of 13kD. Synaptobrevin 2 (VAMP2) is a 116 amino acid protein with an apparent molecular mass of 12.5 kD.
Distribution

Tissue distribution: Nervous system, skeletal muscle and adipose tissue.
Cellular distribution: Synaptic vesicle membrane, cytoplasmic vesicle membrane, and mitochondrion

Function
Synaptobrevin is involved in the targeting and/or fusion of transport vesicles to their target membrane.
Interaction
VAPA, VAPB, Syntaxin, SNAP25
Ligand/Receptor
SNAPs
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Neurotransmitter Receptor Trafficking/Endocytosis, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Gene ID
6843 View all products for this Gene ID
UniProt
View information about Synaptobrevin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06.21.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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