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Purified anti-Syntrophin Antibody

Pricing & Availability
Clone
1351 (See other available formats)
Regulatory Status
RUO
Other Names
Dystrophin-associated protein A1 acidic component 1, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, TACIP1, Syntrophin-1, SNT1, LQT12,dJ1187J4.5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1351_PURE_Syntrophin_Antibody_WB_090915
Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
  • 1351_PURE_Syntrophin_Antibody_WB_090915
    Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
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845102 100 µg 335 CHF
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Description

Syntrophins are a family of five 60 kD adapter proteins that use their multiple protein interaction domains (two PHDs and a PDZ) to localize various signaling proteins (kinases, ion/water channels, or NOS) to specific intracellular locations. In neurons, syntrophins are known as the postsynaptic density protein (PSD) that serves to connect neurotransmitter receptors (such as nAChRs) to the cytoskeleton.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, ICC, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope has been mapped to amino acids 108-120 within the a-syntrophin PDZ domain.

Application References

(PubMed link indicates BioLegend citation)
  1. Froehner S, et al. 1987. J. Cell Biol. 104:1633. (WB, IHC)
  2. Gee S, et al. 1998. J. Neurosci. 18:128. (IP)
  3. Ort T, et al. 2001. EMBO 20:4013. (IP, WB)
  4. Adams M, et al. 2001. J. Cell Biol. 155:113. (IF)
  5. Williams M, et al. 1999. J. Cell Biol. 144:1259. (IHC)
Product Citations
  1. Martin CA, et al. 2022. Regen Biomater. 9:rbac031. PubMed
RRID
AB_2566133 (BioLegend Cat. No. 845102)

Antigen Details

Structure
Monomer and homodimer. Syntrophins consist of a conserved domain, two pleckstin homology domains, a PDZ domain, and a highly conserved C-terminal syntrophin unique (SU) domain. Expected MW: 58 kD.
Function
Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex.
Interaction
Interacts with the other members of the syntrophin family SNTB1 and SNTB2; SGCG and SGCA of the dystrophin glycoprotein complex; NOS1; GRB2; the sodium channel proteins SCN4A and SCN5A; F-actin and calmodulin; Interacts with MYOC.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
6640 View all products for this Gene ID
UniProt
View information about Syntrophin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09.17.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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