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Purified anti-TAB1 Antibody

Pricing & Availability
Clone
W15153A (See other available formats)
Regulatory Status
RUO
Other Names
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, Mitogen-activated protein kinase, kinase 7-interacting protein 1 (MAP3K7IP1), TGF-beta-activated kinase 1-binding protein 1, TAK1-binding protein 1, 3'-Tab1
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15153A_PURE_TAB1_Antibody_1_050218
Total lysates (15 µg protein) from 293T and 293T/TAB1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-TAB1 antibody, clone W15153A (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W15153A_PURE_TAB1_Antibody_1_050218
    Total lysates (15 µg protein) from 293T and 293T/TAB1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-TAB1 antibody, clone W15153A (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W15153A_PURE_TAB1_Antibody_WB_110818
    Total cell lysates (15 µg total protein) HeLa, Jurkat and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Tab Antibody, clone W15153A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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687902 100 µg 229 CHF
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Description

TAB1 (TGFß-Activated Kinase 1-Binding Protein 1), also known as TAK1-Binding Protein 1, is a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which mediates MAPK signaling pathways and proinflammatory signaling induced by TGF-β, IL-1, TNF-α and WNT-1. In the signaling pathway triggered by TGF- β, the binding of TAB1 and TAK1 triggers TAK1 autophosphorylation and activation. In the response to TNF-α or IL-1, TAK1 binds with  TAB1/TAB2 or TAB1/TAB3 to form distinct complexes. In addition, TAB1 can also activate MAPK14/p38α, which represents an alternative activation pathway upon various stimuli. Alternatively spliced transcript variants which encode distinct isoforms have been reported. On the other hand, TAB1 gene disrupted mice demonstrate reduced TAK1 activity and are embryonic lethal.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TAB1 recombinant protein (255-504 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2617007 (BioLegend Cat. No. 687902)

Antigen Details

Structure
504 amino acids with a predicted molecular weight of 54.6 kD. Contains a PPM-type phosphatase domain.
Distribution

Cytoplasm.

Function
TAB1 is a signaling intermediate involved in several signling pathways induced by TGF-β, IL-1, TNF-α and WNT-1. TAB1 regulates the activity of the MAP kinase kinase kinase MAP3K7/TAK1 and plays an important role in embryogenesis and wound repair.
Interaction
Interacts with XIAP, BIRC7, TRAF6, and MAP3K7.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Lanna A, et al. 2014. Nat. Immunol. 15:965.
2. Yang CY, et al. 2014. J. Immunol. 192:1547.
3. Zhu Y, et al. 2013. Genes Dev. 27:1739.
4. Moreno-García ME, et al. 2013. Mol. Cell Biol. 33:1149.
5. Pathak S, et al. 2012. EMBO J. 31:1394.
6. Liu Q, et al. 2009. Nat. Cell Biol. 11:154.

Gene ID
10454 View all products for this Gene ID
UniProt
View information about TAB1 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 05.02.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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