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Purified anti-TACC3 Antibody

Pricing & Availability
Clone
W18281B (See other available formats)
Regulatory Status
RUO
Other Names
Transforming acidic coiled-coil containing protein 3 , ERIC1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18281B_PURE_TACC3_Antibody_1_121521.png
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL (1:2000 dilution) of purified anti-TACC3 antibody (clone W18281B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
  • W18281B_PURE_TACC3_Antibody_1_121521.png
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL (1:2000 dilution) of purified anti-TACC3 antibody (clone W18281B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
  • W18281B_PURE_TACC3_Antibody_2_121521.png
    ICC staining of purified anti-TACC3 antibody (clone W18281B) on A-431 cells. The cells were fixed with 4% paraformaldehyde, permeabilized with Triton X-100, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with no primary antibody (panel A) or 1.25 µg/mL purified anti-TACC3 antibody (clone W18281B) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
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949401 25 µg 118 CHF
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949402 100 µg 293 CHF
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Description

TACC3, also referred to as transforming acidic coiled-coil containing protein 3 or ERIC1, is involved in the process of mitotic spindle assembly and stabilization. TACC3 forms a complex with ch-TOG/clathrin to stabilize the kinetochore fibers of the mitotic spindle. It acts as an inter-microtubule bridge and maintains kinetochore tension. TACC3 is frequently overexpressed in many cancers and may serve as a therapeutic and prognostic marker in breast cancer. Multiple solid tumors have been described where TACC3 is found as a fusion with various FGFR family members, which results in constitutive receptor tyrosine kinase activity of the FGFR partner.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human TACC3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells fixed with methanol or 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. All three methods were compatible with TACC3 staining.

RRID
AB_2910516 (BioLegend Cat. No. 949401)
AB_2910516 (BioLegend Cat. No. 949402)

Antigen Details

Structure
TACC3 is an 838 amino acid protein with a predicted molecular weight of 90 kD.
Distribution

Ubiquitously expressed/cytoplasm and mitotic spindle assembly

Function
Cell cycle, cell division, mitosis, differentiation
Biology Area
Cell Biology
Molecular Family
Microtubules
Antigen References
  1. Gangisetty, et al. 2004. Oncogene. 23:2559.
  2. Costa R, et al. 2016. Oncotarget. 7:55924.
  3. Ding, et al. 2017. Cytoskeleton. 74:369.
  4. Huo Q, et al. 2021. Front. Genet. 12:640078.
Gene ID
10460 View all products for this Gene ID
UniProt
View information about TACC3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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