- Clone
- W18281B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transforming acidic coiled-coil containing protein 3 , ERIC1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
949401 | 25 µg | 118 CHF | ||||
949402 | 100 µg | 293 CHF |
TACC3, also referred to as transforming acidic coiled-coil containing protein 3 or ERIC1, is involved in the process of mitotic spindle assembly and stabilization. TACC3 forms a complex with ch-TOG/clathrin to stabilize the kinetochore fibers of the mitotic spindle. It acts as an inter-microtubule bridge and maintains kinetochore tension. TACC3 is frequently overexpressed in many cancers and may serve as a therapeutic and prognostic marker in breast cancer. Multiple solid tumors have been described where TACC3 is found as a fusion with various FGFR family members, which results in constitutive receptor tyrosine kinase activity of the FGFR partner.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human TACC3 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone was tested for ICC using HeLa cells fixed with methanol or 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. All three methods were compatible with TACC3 staining.
- RRID
-
AB_2910516 (BioLegend Cat. No. 949401)
AB_2910516 (BioLegend Cat. No. 949402)
Antigen Details
- Structure
- TACC3 is an 838 amino acid protein with a predicted molecular weight of 90 kD.
- Distribution
-
Ubiquitously expressed/cytoplasm and mitotic spindle assembly
- Function
- Cell cycle, cell division, mitosis, differentiation
- Biology Area
- Cell Biology
- Molecular Family
- Microtubules
- Antigen References
-
- Gangisetty, et al. 2004. Oncogene. 23:2559.
- Costa R, et al. 2016. Oncotarget. 7:55924.
- Ding, et al. 2017. Cytoskeleton. 74:369.
- Huo Q, et al. 2021. Front. Genet. 12:640078.
- Gene ID
- 10460 View all products for this Gene ID
- UniProt
- View information about TACC3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All TACC3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TACC3 Antibody | W18281B | WB,ICC |
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