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Purified anti-Tapasin (Tapbp) Antibody

Pricing & Availability
Clone
5D3/Tapasin (See other available formats)
Regulatory Status
RUO
Other Names
TPN, TPSN, TAP-associated protein, Tapbp, Tapa
Isotype
Armenian Hamster IgG
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Product Citations
publications
5D3slashTapasin_PURE_Tapasin_Antibody_1_WB_042017
Total protein extracts (15 µg protein) from Tapasin knock-out MEFs (Lane 1), wild-type MEF (Lane 2), THP-1 (Lane 3), Raw264.7 (Lane 4), NIH3T3 (Lane 5) cells and mouse brain tissue (Lane 6) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose and probed with 0.2 µg/mL (1:2500 dilution) purified anti-Tapasin (Tapbp) (clone 5D3/Tapasin) antibody (upper panel) or GAPDH antibody (lower panel) as a loading control. Proteins were visualized by chemiluminescence detection using a goat anti-Armenian Hamster IgG secondary antibody conjugated to HRP for purified anti-Tapasin (Tapbp) antibody or donkey anti-rabbit IgG antibody conjugated to HRP for GAPDH. Lane M: molecular weight ladder.
  • 5D3slashTapasin_PURE_Tapasin_Antibody_1_WB_042017
    Total protein extracts (15 µg protein) from Tapasin knock-out MEFs (Lane 1), wild-type MEF (Lane 2), THP-1 (Lane 3), Raw264.7 (Lane 4), NIH3T3 (Lane 5) cells and mouse brain tissue (Lane 6) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose and probed with 0.2 µg/mL (1:2500 dilution) purified anti-Tapasin (Tapbp) (clone 5D3/Tapasin) antibody (upper panel) or GAPDH antibody (lower panel) as a loading control. Proteins were visualized by chemiluminescence detection using a goat anti-Armenian Hamster IgG secondary antibody conjugated to HRP for purified anti-Tapasin (Tapbp) antibody or donkey anti-rabbit IgG antibody conjugated to HRP for GAPDH. Lane M: molecular weight ladder.
  • 5D3slashTapasin_PURE_Tapasin_Antibody_2_WB_042017
  • 5D3slashTapasin_PURE_Tapasin_Antibody_3_WB_071018
    Total cell lysates (15 µg protein) from Raw264.7 cells (lane 1) and mouse brain tissue (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.2 µg/mL (1:2500 dilution) of Purified anti-Tapasin (Tapbp) Antibody (clone 5D3/Tapasin) (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Armenian hamster-IgG secondary antibody conjugated to HRP for anti-Tapasin (Tapbp) Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1(lower panel) as a loading control. Lane M: Molecular weight ladder.
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696702 100 µg 288 CHF
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Description

Major histocompatibility complex (MHC) class I molecules present peptide antigens recognized by CD8+ T cells to enable the immune recognition of virally or malignantly transformed cells exposing non-self peptides. Selection and loading of peptides onto MHC I is orchestrated by the peptide-loading complex (PLC). Tapasin, a central component of the PLC, stabilizes MHC I and catalyzes the exchange of low-affinity against high-affinity, immunodominant peptides. In this quality-control step, tapasin cooperates with other more general, ER-resident chaperones, including ERp57 and TAP. Tapasin has been found to be downregulated in glioblastoma, renal cell carcinoma, prostate cancer, urothelial carcinoma, cervical carcinoma, head and neck carcinoma and esophageal squamous cell carcinoma. Studies also reported reduced tapasin to be linked to metastasis and poor outcome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Partial mouse tapasin peptide (11-34 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-reacts with human (in-house tested). This antibody recognizes a non-specific band over 100 kD in some tested cell lysates.

Product Citations
  1. Boulanger DSM, et al. 2022. Front Immunol. 13:956603. PubMed
  2. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
RRID
AB_2687096 (BioLegend Cat. No. 696702)

Antigen Details

Structure
465 amino acids with a predicted molecular weight of 50 kD. An isoform with predicted MW of 47kD was reported.
Distribution

Endoplasmic reticulum membrane

Function
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide.
Interaction
Interacts with TAP1 and is thus a subunit of the TAP complex. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, ERp57 and TAP.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells, Ubiquitin/Protein Degradation
Molecular Family
MHC Antigens
Antigen References
  1.  Neefjes J, et al. 2011. Nat. Rev. Immunol. 11:823-36.
  2. Dong G, et al. 2009. Immunity. 30:21.
  3. Fisette O, et al. 2016. Sci. Rep. 6:19085.
  4. Dick TP, et al. 2002. Immunity. 16: 87.
  5. Ortmann B, et al. 1997. Science. 277: 1306.
  6. Thuring C, et al. 2015. Br. J. Cancer. 113:952.  
  7. Han LY, et al. 2008. Clin. Cancer Res. 14: 3372.
  8. Seliger B, et al. 2010. Cancer Immunol. Immunother. 59: 529.
Gene ID
21356 View all products for this Gene ID
UniProt
View information about Tapasin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07.10.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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